霍恩氏法与序贯法在急性经口毒性试验中应用研究

[目的]探讨序贯法作为农药急性毒性替代方法的可靠性、可操作性以及该方法的优缺点。[方法]采用序贯法与霍恩氏法分别测定6种农药制剂的急性经口半数致死剂量(LD50)和95%可信区间,比较2种方法获得的急性毒性分级和试验所用动物数量。[结果]序贯法测得的LD50与霍恩氏法相近,毒性分级与霍恩氏法均一致;但序贯法试验使用动物数量减少了52.3%。[结论]序贯法测定农药急性经口LD50具有明显优势,用于农药急性经口毒性检测具有广阔前景。

Application of Horn's and Dixon-Mood in Acute Oral Toxicity Test of Pesticides

[Objective] The aim was to explore Dixon-Mood as pesticide acute toxicity alternative methods of reliability and operability and the advantages and disadvantages of this method.[Method] The median lethal dose (LD50) and 95% confidence intervals of 6 pesticides were detected by Dixon-Mood and Horn''s,and the two acute toxicity classifications and the animal consumption of the two methods were compared.[Result] The LD50 and 95% confidence intervals of the Dixon-Mood were similar with those of the Horn''s,and the animals could be economized by 52.3% with the Dixon-Mood compared with those of the Horn''s.[Conclusion] The Dixon-Mood determination of pesticide acute tramsotal LD50 has obvious advantages,and has wide prospect in the pesticide acute tramsotal toxicity testing.