| 赛加羚羊的分子生物学鉴别 |
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| 引用本文： | 张晓璐,白素英,徐艳春.赛加羚羊的分子生物学鉴别[J].东北林业大学学报,2006,34(3):106-108. |
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| 作者姓名： | 张晓璐 白素英 徐艳春 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 黑龙江省科技攻关项目;黑龙江省杰出青年科学基金 |
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| 摘 要： | 为了解决以往赛加羚羊(Saiga tatarica)鉴别方法存在的不确定性或分辨率低的问题,在mtDNA的12S rRNA基因易变区设计了2对赛加羚羊特异性引物Saiga A和Saiga B,通过PCR扩增和常规电泳检测,分别得到127 bp和320 bp的片段,进而可通过这两个片段的有无来特异性检出赛加羚羊的DNA。引物Saiga A和Saiga B的最小检出量为1．0 ng DNA,稳定性和特异性好、灵敏度很高,可以应用于赛加羚羊样品的检测。
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| 关 键 词： | 赛加羚羊 Saiga tatarica 12S rRNA 物种鉴定 |
| 收稿时间： | 2005-05-18 |
| 修稿时间： | 2005-05-18 |
Molecular Biological Identification of Saiga |
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| Zhang Xiaolu,Bai Suying,Xu Yanchun.Molecular Biological Identification of Saiga[J].Journal of Northeast Forestry University,2006,34(3):106-108. |
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| Authors: | Zhang Xiaolu Bai Suying Xu Yanchun |
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| Institution: | Wildlife Resources College of Northeast Forestry University, Harbin 150040, P. R. China |
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| Abstract: | Two saiga-speeific primer pairs,Saiga A and Saiga B,were designed at the variable region of 12S rRNA gene of mito- chondfial genome aiming to solve the uncertainty or low resolution for already existed methods.A 127 bp and a 320 bp frag- ment were obtained respectively from these two primer pairs through PCR amplification.Then saiga DNA could be identi- fied by the appearance of the two fragments through routine electrophoresis.The lowest positive quantity of template DNA was 1.0 ng for the two primer pairs.Results above indicate that this method with high stability,specificity and sensitivity can be applied to sample identification for saiga. |
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| Keywords: | Saiga Saiga tatarica 12S rRNA Species identification |
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