| 24株剑麻根际联合固氮菌的16S rDNA序列分析 |
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| 引用本文： | 张世清,李庆洋,高建明,陈河龙,郑金龙,刘巧莲,易克贤.24株剑麻根际联合固氮菌的16S rDNA序列分析[J].江西农业学报,2011(11):132-134. |
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| 作者姓名： | 张世清 李庆洋 高建明 陈河龙 郑金龙 刘巧莲 易克贤 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室;海南大学农学院;中国热带农业科学院环境与植物保护研究所; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(ITBB110210); 国家麻类现代产业技术体系建设专项(CARS-19-E17); 引进国外技术管理人才项目(20114600031) |
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| 摘 要： | 对海南、广西和广东剑麻主产区种植基地的剑麻根际联合固氮菌进行了分离、纯化及固氮活性测定,获得了24株具有固氮活性的菌株,其固氮酶活性为5.8742～1765.6590 nmol/(mL.h)。16S rDNA序列分析结果表明:参试菌株的16S rDNA扩增区约为1500 bp,无太大的长度变异,其系统发育树表明这24株菌株分别属于6个不同的属。
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| 关 键 词： | 剑麻 根际 联合固氮菌 16S rDNA |
16S rDNA Sequence Analysis of 24 Strains of Associative Nitrogen Fixation Bacteria from Rhizosphere of Sisal |
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| ZHANG Shi-qing,LI Qing-yang,GAO Jian-ming,CHEN He-long,ZHENG Jin-long,LIU Qiao-lian,YI Ke-xian.16S rDNA Sequence Analysis of 24 Strains of Associative Nitrogen Fixation Bacteria from Rhizosphere of Sisal[J].Acta Agriculturae Jiangxi,2011(11):132-134. |
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| Authors: | ZHANG Shi-qing LI Qing-yang GAO Jian-ming CHEN He-long ZHENG Jin-long LIU Qiao-lian YI Ke-xian |
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| Institution: | ZHANG Shi-qing1,3,LI Qing-yang3,GAO Jian-ming1,CHEN He-long1,ZHENG Jin-long1,LIU Qiao-lian1,YI Ke-xian1,2(1.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Key and Opening Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Haikou 571101,China,2.Institute of Environment and Plant Protection,Danzhou 571737,3.College of Agronomy,Hainan University,China) |
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| Abstract: | Through the separation and purification of associative nitrogen fixation bacteria,as well as the determination of their nitrogenase activities by using acetylene reduction method,twenty-four bacterial strains with nitrogen fixation activity were obtained from the rhizosphere of sisal plants,which were planted in the main production area of Hainan,Guangxi and Guangdong.Their nitrogenase activities varied from 5.8742 to 1765.6590 nmol/（mL·h）.The results of sequence analysis of 16S rDNA showed that the amplification section of 16S rDNA from the tested strains had no large changes in the length,and it was about 1500 bp.The 24 strains belonged to 6 different genera,through searching their homologic sequences in the GenBank and constructing their phylogenetic tree. |
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| Keywords: | Sisal Rhizosphere Associative nitrogen fixation bacteria 16S rDNA |
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