应用种子进行穿龙薯蓣组织培养的研究

以穿龙薯蓣种子为外植体诱导出了愈伤组织,最佳培养基为MS 6-BA3.0mg/L NAA2.0mg/L,最高出愈率为18.9%;诱导不定芽的最佳培养基为MS 6-BA2.0mg/L NAA2.0mg/L,最高诱导率可达75.5%;最佳壮苗培养基为1/2MS 6-BA 1.0mg/L KT1.0m g/L;根诱导的最佳培养基为1/2MS 6-BA0.5mg/L NAA0.5mg/L,诱导率最高达86.2%;最佳的炼苗基质为腐质土∶蛭石∶河沙=1∶1∶1,成活率最高达84.8%。

Using Seeds as Explant on Dioscorea nipponica Makino. Tissue Culture

Callus was induced using seeds as explant on Dioscorea nipponica Makino,the best culture media is MS 6-BA3.0 mg/L NAA2.0 mg/L and the highest frequency is 18.9%.The gemma can be induced when the callus was transferred into the culture medium with MS 6-BA2.0mg/L NAA2.0mg/L and the highest inducement frequency can be reach 75.5%.The buds grow strongly on the culture medium with 1/2MS 6-BA1.0mg/L KT1.0mg/L and radicate easily on the culture medium with 1/2MS 6-BA 0.5mg/L NAA 0.5mg/L.Radication frequency can be reach 86.2%.The transplanting medium in rotten quality soil,vermiculite and river sand by 1 to 1 to 1,is useful,84.8% seedlings can survive if the technique is fine.