陕西省猪伪狂犬病流行病学调查和WG株的分离鉴定

应用gE-EL ISA法对陕西省部分地区6个集约化猪场的187份猪血清进行了伪狂犬病毒(P seu-dorab ies v irus,PRV)野毒感染检测,并对疑似伪狂犬病发病仔猪的内脏及脑组织进行了病毒分离鉴定。结果显示,陕西省猪PRV野毒抗体平均阳性率为28.76%,最高为48.98%,最低为0;分离毒株可致PK-15细胞出现典型的圆缩、集聚,脱落等细胞病变,连续传代后,CPE出现的时间稳定在20 h左右;细胞培养物和病料混悬液接种家兔均出现典型奇痒症状,PCR反应扩增出PRV gE基因中长度为612 bp的特异性片段。表明本试验分离的病毒为PRV,并将其命名为PRV W G株。

Isolation of pseudorabies virus Shaanxi WG strain and epidemiology investigation

About 187 serum samples from 6 pig farms in some regions of Shaanxi province were detected with the gE-ELISA Kit which can distinguish the vaccination from wild-type virus infection,and porcine pseudorabies virus was isolated from the purtenance and brain of a 2-day-old pig and then the virus was identified microbiologically. The results showed that the average positive rate was 28.76% ,the highest gEantibody of wild-type PRV was 48.98% and the lowest was 0;It infected PK-15 cell line and caused it to shrink, aggregate, and detach from the culture system in 20 h approximately, and the inoculated rabbits showed classical clinical signs when challenged with the culture and positive material. Then the specific 612 bp bands by PCR of the isolated virus were obtained. These data indicate that the isolated virus was PRV and referred to as WG strain in this report.