朱砂叶螨抗药性与羧酸酯酶活性的关系

羧酸酯酶是昆虫体内普遍存在的一种水解酶。昆虫对多数有机磷、氨基甲酸酯及有些菊酯类杀虫剂产生抗性羧酸酯酶活性有关1～2]。桃蚜(Muscapersicae)对有机磷的抗性与其体内所含酯酶活力呈正相关,E4酯酶的量随着抗性程度的增加而呈几何级数上升3]。棉蚜(Aphisgossypii)对对硫磷和对氧磷产生抗性后,α-乙酸萘酯酶活力分别增加15.25倍和60.30倍4]。本文研究了朱砂叶螨(Tetranychuscinnabarinus)对氧化乐果(有机磷)、三氯杀螨醇(有机氯)、双甲脒(有机氮)及哒螨灵(杂环类)的抗性与羧酸酯酶的关系。1　材料与方法1.1　供试叶螨在人工气象室内…

RELATIONSHIP BETWEEN OF CARBOXYLESTERASE ACTIVITY AND RESISTANCE OF TETRANYCHUS CINNABARINUS (ACARI: TETRANYCHIDAE) TO PESTICIDES

This paper reported that carboxylesterase activity of the resistant strains is notably higher than that of the susceptible strain, and observable relation with resistant factor. Factor of the resistance to omethoate, dicofol, amitraz and pyridaben on -Tetranychus cinnabarinus- were 152.83, 55.59, 62.61, and 15.67 folds, respectively. The relative specific activity of the carboxylesterase in the four strains were 3.52, 2.60, 3.24, and 1.73, respectively, and up while the factor is on the increase. Three rotational spraying strains, dicofol and amitraz, dicofol and omethoate, omethoate and amitraz in turn the resistant level to relative pesticides and relative specific activity were lower than that of spraying one pesticide to a batch. The relative specific activity of carboxylesterase were 2.06, 2.52, and 2.41, respectively. These results showed that increasing of the carboxylesterase activity is a one of biochemistry mechanisms of development of resistance to omethoate, dicofol, amitraz, pyridaben in -T. cinnabarinus.- In addition, the larger activity of the enzyme, the higher resistance folds on the spider mite.