内蒙古绒山羊褪黑激素受体1a基因外显子1 克隆及序列分析

参考GenBank上已发表的绵羊(登录号:15U14109)、人(登录号:U14108)、牛(登录号:U73327)、鼠(登录号:U52222)等物种褪黑激素受体(melatonin receptor 1a,MTNR1a)基因 cDNA序列设计引物,以内蒙古绒山羊基因组DNA为模板进行PCR扩增,得到257 bp的基因片段,将扩增产物进行克隆测序后与GenBank数据库进行序列同源性比较。结果表明,绒山羊MNTR1a基因外显子1序列与已发表的绵羊和牛该基因序列同源性分别为99%和96%,说明所得到的序列为绒山羊MNTR1a基因的外显子1序列。利用DNAStar软件分析,得到该基因序列的257个核苷酸。该序列包括5''UTR 23个核苷酸、翻译起始密码子ATG和N端78个氨基酸编码序列。

Cloning and Sequence Analysis of Exon1 MTNR1a Gene of Inner Mongolian Cashmere Goat

According to MTNR1a cDNA sequence of sheep(gi:15U14109), human(gi:U14108), cattle(gi:U73327), mice(gi:U52222) and other species announced in GenBank,primers were designed. The 275 bp fragment of exon 1 of MTNR1a gene was amplified by PCR in Inner Mongolian Cashmere goat. The PCR products were cloned and sequenced. By compared with database of GenBank, it turned out that homology of nucleotide sequence of exon 1 MTNR1a gene in Cashmere goat with sheep, cow were 99% and 96%, respectively. It showed that the sequence was MTNR1a gene exon 1 sequence in Cashmere goat. Using DNAStar software to analysis, 257 nucleotides of the gene sequence was gotten.The sequence contained 5''UTR 23 nucleotides, translation initiation codon ATG and the N terminal 78 amino acid coding sequences.