| 家兔MC1R基因5'-侧翼序列的克隆及测序 |
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| 引用本文： | 罗浩,侯佳妮,李玉梅,闫守庆.家兔MC1R基因5'-侧翼序列的克隆及测序[J].中国畜牧兽医,2011,38(5):89-92. |
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| 作者姓名： | 罗浩 侯佳妮 李玉梅 闫守庆 |
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| 作者单位： | 吉林大学畜牧兽医学院,吉林长春 130062 |
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| 摘 要： | 毛色作为家兔的一种遗传标记,在品种特征、纯度鉴定及品种选育中均具有重要的作用。MC1R基因作为控制动物毛色的重要基因之一,其核苷酸序列多态性与多种动物的毛色表型相关。本试验根据GenBank中已知的兔MC1R基因核苷酸序列(登录号:AM180881)设计引物RMCR1和RMCR2,采用PCR-末端加尾法获得了兔MC1R基因5'端930 bp的未知序列,该序列与GenBank数据库中的已知序列同源性为99%。
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| 关 键 词： | 家兔 毛色 MC1R基因 |
| 收稿时间： | 2010-11-15 |
Cloning and Sequence of MC1R Gene 5'-Flanking Sequence in Rabbit |
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| LUO Hao,HOU Jia-ni,LI Yu-mei,YAN Shou-qing.Cloning and Sequence of MC1R Gene 5'-Flanking Sequence in Rabbit[J].China Animal Husbandry & Veterinary Medicine,2011,38(5):89-92. |
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| Authors: | LUO Hao HOU Jia-ni LI Yu-mei YAN Shou-qing |
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| Institution: | College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China |
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| Abstract: | The rabbit coat color as one of available genetic markers plays an important role in determining breed characteristics, purity, and crossing combination. The melanocortin-1 receptor gene (MC1R) is an important candidate gene for coat color heredity because some coat color changes in many species resulted from the nucletide sequence variants of MC1R gene. In this study,two primers (RMCR1 and RMCR2) were designed to amplify the 5''-flanking region sequences of MC1R from rabbit genome according to the known rabbit MC1R sequence(AM180881). A DNA fragment of 930 bp in length was cloned from 5''-flanking sequence of MC1R gene by an end tailed-PCR strategy, the homology of nucleotide was 99% with the known rabbit MC1R sequence(AM180881). |
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| Keywords: | rabbit coat color MC1R gene |
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