不同水平的氨基酸对小香鸡免疫性能与抗氧化性能的影响

选取120只健康、体重相近、出栏前5周的商品小香鸡为试验动物,随机分为苏氨酸、异亮氨酸、亮氨酸、缬氨酸4个处理,每周称重1次。屠宰时,每个重复选取具有代表性的公、母鸡各1只,进行翅下静脉采血,分别测定免疫指标、抗氧化性能指标。结果表明,3种水平的苏氨酸处理对小香鸡免疫、抗氧化性能之间的差异不显著;添加0.3%的异亮氨酸比添加0.1%的异亮氨酸能显著提高小香鸡抗氧化性能;添加0.2%的异亮氨酸比添加0.1%异亮氨酸能显著提高总蛋白含量;添加0.2%的缬氨酸较添加0.3%缬氨酸能显著提高GSH-PX活性;添加0.2%的缬氨酸与添加0.1%的缬氨酸相比,总蛋白含量显著提高;添加0.3%的缬氨酸与添加0.1%的缬氨酸、0.2%的缬氨酸相比,总蛋白含量差异不显著;添加0.2%的缬氨酸处理的球蛋白含量与添加0.1%的缬氨酸、0.3%的缬氨酸差异显著;苏氨酸处理组与亮氨酸处理组球蛋白含量差异极显著,异亮氨酸处理组与亮氨酸处理组球蛋白含量差异显著,但是缬氨酸处理组球蛋白含量与其他3组差异均不显著。

Effects of Different Levels of Amino Acids on Immunity and Antioxidation of Xiaoxiang Chicken

One hundred and twenty healthy and body weight-alike Xiaoxiang chickens,which will be sold from the farm after five weeks,were selected as the experimental animals,the ratio of cock to hen was 1∶1,they were randomly divided into Thr,Leu,Val and Ile group,each group had 3 repeats,and every repeat tested 10 chickens.The pretesting period was 2 days,the testing period was 28 days,and the tested chickens were weighed every week.A pair of typical cock and hen in each repeat were selected to pick the blood under their wing vein when they were slaughtered,and their immune indexes and antioxidative indexes were determined.The results of the experiment showed that there was no significant difference in the immunity and antioxidation of Xiaoxiang chicken among three levels of Thr treatments.Comparing with adding 0.1% Ile,adding 0.3% Ile could significantly improve the antioxidation,and adding 0.2% Ile could significantly enhance the total protein content.In comparison with adding 0.3% Val,adding 0.2% Val could markedly increase the vitality of GSH-PX.Adding 0.2% Val could significantly improve the total protein content as compared with adding 0.1% Val,while there was no significant difference in total protein content between adding 0.3% Val and adding 0.1% Val or 0.2% Val.The globulin content of adding 0.2% Val was significantly different from that of adding 0.1% Val or 0.3% Val.For globulin content,there was extremely significant difference between Thr group and Leu group,significant difference between Ile group and Leu group,no significant difference between Val group and other three groups.