Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (<Emphasis Type="Italic">Verasper variegatus</Emphasis>) |
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Authors: | Xiang-Shan Ji Song-Lin Chen Jing-Feng Yang Hong-Yu Ma Yun-Liang Jiang |
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Institution: | (1) Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, 266071 Qingdao, China;(2) College of Animal Science and Technology, Shandong Agricultural University, 271018 Taian, China; |
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Abstract: | In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio
of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic
population was assessed as opposed to cultivated population. The results showed that high percentage of meiotic gynogenetic
diploids were generated when the eggs fertilized with irradiated heterologous sea perch frozen sperm (30–50 mJ cm−2) were cold shocked in sea water of −1°C for 40–75 min at 5 min after fertilization. About 15,200 diploid gynogenetic larvae
were achieved and they exhibited normal morphology similar to diploid control. The gynogenetic diploids were 100% female,
which first confirmed the female homogamete (XX/XY) sex determination in spotted halibut. The genetic analysis showed that
the average H
O
was, respectively, 0.404 and 0.724 in gynogenetic population and cultivated population, indicating an increase of homozygosity
in gynogenetic population. |
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Keywords: | |
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