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| 运用MTT比色法检测印楝素对果蝇S2细胞的毒力作用 | |
| 引用本文： | 徐霖,杨明飞,冉雪琴,王嘉福. 运用MTT比色法检测印楝素对果蝇S2细胞的毒力作用[J]. 河南农业大学学报, 2012, 46(1): 72-75 |
| 作者姓名： | 徐霖 杨明飞 冉雪琴 王嘉福 |
| 作者单位： | 1. 贵州大学动物科学学院,贵州,贵阳,550025;贵阳学院生物与环境工程系,贵州,贵阳,550005 2. 贵阳护理职业学院,贵州,贵阳,550081 3. 贵州大学动物科学学院,贵州,贵阳,550025;贵州大学农业生物工程省重点实验室,贵州,贵阳,550025 |
| 摘 要： | 采用MTT(四甲基偶氮唑蓝,methylthiazolyl-tetrazolium)法测定不同质量浓度印楝素(0.01,0.1,0.5,1.0,2.0mg·L-)作用不同时间(24,48,72,96h)对果蝇S2细胞的抑制率.结果表明,印楝素低质量浓度处理时,对果蝇S2细胞有促进生长的作用,表现为低质量浓度促进效应；在质量浓度为0.1～2mg·L-1处理剂量范围内,表现为明显的时间依赖性,随时间延长,抑制率增加.0.5mg·L-1处理48h抑制率达54％,为最佳作用条件. |
| 关 键 词： | 印楝素 果蝇S2细胞 细胞活性 MTT比色法 |
Testing the toxicity of azadirachtin on Drosophila S2 cell with MTT method |
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| XU Lin , YANG Ming-fei , RAN Xue-qin , WANG Jia-fu. Testing the toxicity of azadirachtin on Drosophila S2 cell with MTT method[J]. Journal of Henan Agricultural University, 2012, 46(1): 72-75 | |
| Authors: | XU Lin YANG Ming-fei RAN Xue-qin WANG Jia-fu |
| Affiliation: | 1,4 (1.College of Animal Science,Guizhou University,Guiyang 550025,China; 2.The Department of Biology and Environment engineering,Guiyang College,Guiyang 550005,China; 3.Guiyang Nursing Vocational,Guiyang,550081,China; 4.Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China) |
| Abstract: | The inhibitory infection of Drosophila S2 cell lines treated with different concentration 0.01,0.1,0.5,1.0,2.0 mg·L-1of azadirachtin for different times(24,48,72,96 h)were determined by MTT(methylthiazolyl-tetrazolium) method.There had obvious activating effect at low concentration but inhibiting effect at higher one.All infects were in a time-depent manner.The inhibitory ratio is 54% when the disposal condition is 0.5 mg·L-1 and 48 h. |
| Keywords: | azadirachtin Schneider’s Drosophila S2 cell activity methylthiazolyl-tetrazolium method |
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