| 烟草DNA的提取与SRAP反应体系优化 |
| |
| 引用本文： | 徐建华,杨虹琦,杨程,周冀衡,段凤云,陈信波,詹莜国,赵世浩. 烟草DNA的提取与SRAP反应体系优化[J]. 湖南农业大学学报(自然科学版), 2009, 35(3) |
| |
| 作者姓名： | 徐建华 杨虹琦 杨程 周冀衡 段凤云 陈信波 詹莜国 赵世浩 |
| |
| 作者单位： | 1. 湖南农业大学,烟草科学与健康重点实验室,湖南,长沙,410128
2. 云南省大理州烟草公司,技术中心,云南,大理,671000 |
| |
| 基金项目： | 云南省大理自治州烟草公司项目 |
| |
| 摘 要： | 以8个烤烟栽培品种为试验材料,对烟草基因组DNA的提取方法进行比较,并对建立烟草SRAP-PCR 反应体系的影响因子设置梯度试验.结果显示:改进的CTAB法能较好地满足烟草基因组DNA的提取,在20 μL SRAP-PCR的反应体系中,DNA模板40 ng、Mg2+1.5 mmol/L、dNTP 0.25 mmol/L、引物0.2 μmol/L、Taq聚合酶1.5 U,8个烤烟品种的扩增多态性高,带型清晰,差异明显.
|
| 关 键 词： | 烟草 DNA提取 优化 |
DNA extraction and optimization of SRAP reaction system in tobacco |
| |
| XU Jian-hua,YANG Hong-qi,YANG Cheng,ZHOU Ji-heng,DUAN Feng-yun,CHEN Xin-bo,ZHAN You-guo,ZHAO Shi-hao. DNA extraction and optimization of SRAP reaction system in tobacco[J]. Journal of Hunan Agricultural University, 2009, 35(3) |
| |
| Authors: | XU Jian-hua YANG Hong-qi YANG Cheng ZHOU Ji-heng DUAN Feng-yun CHEN Xin-bo ZHAN You-guo ZHAO Shi-hao |
| |
| Affiliation: | 1.Key Laboratory of Tobacco Science and Healthy;HNAU;Changsha 410128;China;2.Technology Center Tobacco Dali Corp;Dali;Yunnan 671000;China |
| |
| Abstract: | Method of DNA extraction and factors influencing SRAP analysis were studied using eight tobacco cultivars. The results showed that,improved method of CTAB contributed to a better extraction of genomic DNA of tobacco. A reliable PCR reaction system for detecting SRAP was developed,each 20 μL PCR reaction mixture consisted of 40 ng of genomic DNA,0.25 mmol/L of dNTPs,1.5 mmol/L of Mg2+,0.2 μmol/L of primer and 1.5 unit of Taq polymerase,every amplification out of the eight cultivars was effective,clear and their differences were significant. |
| |
| Keywords: | SRAP tobacco DNA extraction sequence related amplified polymorphism optimization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
