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| 茶黄素、儿茶素和绞股蓝皂苷复方制剂降脂作用研究 | |
| 引用本文： | 张聪,谭蓉,孔俊豪,葛庆丰,杨秀芳,汪志君. 茶黄素、儿茶素和绞股蓝皂苷复方制剂降脂作用研究[J]. 茶叶科学, 2014, 34(4): 401-407. DOI: 10.13305/j.cnki.jts.2014.04.013 |
| 作者姓名： | 张聪 谭蓉 孔俊豪 葛庆丰 杨秀芳 汪志君 |
| 作者单位： | 1. 扬州大学食品科学与工程学院,江苏 扬州 225127; 2. 中华全国供销合作总社杭州茶叶研究院,浙江 杭州 310016; 3. 浙江省茶资源跨界应用技术重点实验室,浙江 杭州 310016 |
| 基金项目： | 浙江省公益技术研究农业项目（2012C22093） |
| 摘 要： | 以1 mmol·L-1油酸孵育HepG2细胞24 h,建立体外高脂模型,在细胞存活率实验（MTT法）的基础上,以脂质清除率为指标,评价茶黄素（TFs）、儿茶素（Cs）和绞股蓝皂苷（SPs）的体外降脂效果;并结合Box-Behnken响应曲面法（RSM）对3种物质体外联合降脂的浓度进行优化。结果表明：茶黄素、儿茶素和绞股蓝皂苷均能清除肝细胞内堆积的脂肪,若三者同时作用,协同效果明显,能起到防治高血脂症的作用。 |
| 关 键 词： | 茶黄素 儿茶素 绞股蓝皂苷 体外 降血脂 |
| 收稿时间： | 2013-12-11 |
Research on the Antihyperlipdemic Activity of Theaflavins,Catechins and Saponius Mixture Preparations |
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| ZHANG Cong,TAN Rong,KONG Junhao,GE Qingfeng,YANG Xiufang,WANG Zhijun. Research on the Antihyperlipdemic Activity of Theaflavins,Catechins and Saponius Mixture Preparations[J]. Journal of Tea Science, 2014, 34(4): 401-407. DOI: 10.13305/j.cnki.jts.2014.04.013 | |
| Authors: | ZHANG Cong TAN Rong KONG Junhao GE Qingfeng YANG Xiufang WANG Zhijun |
| Affiliation: | 1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; 2. Hangzhou Tea Research Institute, CHINA COOP, Hangzhou 310016, China; 3. Zhejiang key laboratory of transboundary applied technology for tea resources, Hangzhou 310016, China |
| Abstract: | The antihyperlipdemic activity in vitro of theaflavins(TFs), catechins(Cs) and saponius(SPs) were assessed in lipid clearance rate in the model of HepG2 steatosis by using the admini stration with 1 mmol·L-1 oleic acid(OA) in 24 h. Based on the cell viability test(MTT value), lipid clearance was used to evaluate the effect of TFs, Cs and SPs on HepG2 steatosis. The response surface methodology based on a Box-Behnken experimental design was used to optimize the density of the three extractions for combined lipid-lowering in vitro. Results indicated that TFs, Cs and SPs showed a significant inhibitory effect on TG accumulation in HepG2 cells. The ability of lipid-lowering was significant promoted when the three extractions acted on HepG2 at the same time. TFs, Cs and SPs could showed the prevention and curing effect of hyperlipidemia. |
| Keywords: | theaflavins catechins saponius |
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