| 普洱茶挥发性成分指纹图谱研究 |
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| 引用本文： | 吕海鹏,钟秋生,施江,张悦,林智.普洱茶挥发性成分指纹图谱研究[J].茶叶科学,2014,34(1):71-78. |
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| 作者姓名： | 吕海鹏 钟秋生 施江 张悦 林智 |
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| 作者单位： | 1. 农业部茶树生物学与资源利用重点实验室;中国农业科学院茶叶研究所 浙江 杭州 310008; 2. 福建省农业科学院茶叶研究所 福建 福安 355000 |
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| 基金项目： | 国家自然科学基金（31000317）、云南省创新办科技计划课题（2007YNCXB-01-01） |
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| 摘 要： | 采用顶空固相微萃取-气相色谱-质谱（HS-SPME/GC-MS）技术对普洱茶挥发性成分的指纹图谱进行了研究。结果表明,该技术可使普洱茶的挥发性成分得到良好的分离;通过对17个样品的GC图谱的分析,共确定了26个色谱峰为普洱茶挥发性成分的特征指纹峰,建立了普洱茶挥发性成分的GC-MS指纹图谱,并对指纹图谱进行了相似度分析。该方法重复性好,挥发性成分中各成分分离较好,因此所建立的指纹图谱可作为普洱茶香气品质鉴定和评价的参考依据之一。
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| 关 键 词： | 普洱茶 挥发性成分 顶空固相微萃取-气相色谱-质谱 指纹图谱 |
| 收稿时间： | 2013-04-22 |
Study on Fingerprint on Volatile Constituents of Pu-erh Tea |
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| LV Haipeng,ZHONG Qiusheng,SHI Jiang,ZHANG Yue,LIN Zhi.Study on Fingerprint on Volatile Constituents of Pu-erh Tea[J].Journal of Tea Science,2014,34(1):71-78. |
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| Authors: | LV Haipeng ZHONG Qiusheng SHI Jiang ZHANG Yue LIN Zhi |
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| Institution: | 1. Key Lab of Tea Biology and Resources Utilization, Ministry of Agricultry, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 2. Tea Research Institute, Academy of Agricultural Sciences of Fujian, Fu’an 355000, China |
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| Abstract: | The fingerprint on volatile constituents of Pu-erh tea was investigated using HS-SPME/GC-MS. Results showed that the volatile constituents of Pu-erh tea can be well separated using HS-SPME/GC-MS. Based on the analysis of GC chromatogram of 17 tea samples collected from Yunnan province, it was confirmed that 26 peaks in chromatograms can be selected as the fingerprint of peaks of volatile constituents, and it was established as the fingerprint chromatogram for Pu-erh tea. Moreover, the GC fingerprints were analyzed by similarity analysis. The method is of high degree of reproducibility and the resolving of each component is satisfactory, thus the established fingerprints could be used as one of the aroma quality control index for identification and quality assessment of Pu-erh tea. |
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| Keywords: | Pu-erh tea volatile constituents HS-SPME/GC-MS fingerprint |
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