小麦质不育系花药及雌蕊邻苯二酚氧化酶同工酶分析

小麦细胞质雄性不育系、保持系花药三个不同发育时期的邻苯二酚氧化酶同工酶存在明显差异,不育系中的同工酶谱带数多于保持系.考虑到小麦细胞质雄性不育系的细胞核与保持系相同,但具有与保持系不相同的不育细胞质,故邻苯二酚氧化酶同工酶可能与植物的雄性不育特性有某种联系.雌蕊中的邻苯二酚氧化酶同工酶在不育系与保持系间基本相同,这可能与雌蕊中不育系、保持系间的核质组成相同有关,这一事实似乎从另一侧面说明了邻苯二酚氧化酶与不育特性存在着某种联系.

A Comparative Electroporetic Analysis on Catechol Oxidase Isozymes of Anther and Pistil of Cytoplasmic Male-sterile Line and Maintainer in T.aestivum

In this study the catechol oxidase isozymes relative to cytoplasmic male-sterile character were analysed in wheat. The results showed that the obvious differences of catechol oxidase isozyme were observed at different anther development stages of cytoplasmic male-sterile line and maintainer line in wheat. The catechol oxidase isozyme bands of male-sterile line were more than that of maintainer line. 12 isozyme bands (xylE1-xylE12) appeared in cytoplasmic male-sterile line (65C/474A, Shi 63 r4A) , but 10-11 isozyme bands (xy1E1-xylEl0, xylE1-xylE11were present in maintainer line (65C/ 474B, Shi 63 r4B) . xylE4 may be a basic band because it was present in two cytoplasmic male-sterile lines and maintainer lines of wheat.The pistil catechol oxidase isozymes of male-sterile line and maintainer line were approximately same. It is indicated that it may be a normal fertility of pistil in male-sterile line and maintainer line.In all developmental stages of anther the difference of catechol oxidase isozyme can be examined. It may be a genetic constitutionally uncoordinated action in male-sterile line.From these results it was proposed that certain relation between catechol oxidase isozyme and male-sterile character may be present.