布鲁菌疫苗株Rev.1全基因序列的测定与分析

为促进布鲁菌病Rev.1疫苗及相关疫苗的研发, 该研究提取Rev.1疫苗株核酸, 应用PacBio平台进行全基因序列测定与分析。结果表明, Rev.1疫苗株基因组大小约3 299 187 bp, G+C含量为57.2%, 组装为染色体1、染色体2两条环状基因组, 大小分别为2 121 370、1 177 817 bp, G+C含量分别为57.2%、57.3%。将其Ery、BLS和VirB10基因序列与9株GenBank上发表的布鲁菌参考菌株的Ery、BLS和VirB10基因序列进行比较分析, 存在不同程度的差异, 同源性为97.4%~100%。

Genome-wide Sequencing and Analysis for Brucella Rev.1Vaccine Strain

In order to promote the research and development of Brucella Rev.1 vaccine and related vaccines, the nucleic acid of the Brucella Rev.1 vaccine strain was extracted, and the genome-wide sequencing for Brucella Rev.1 vaccine strain was carried out and analyzed through PacBio platform. The results showed that the full-length genome of the Brucella Rev.1 vaccine strain was 3 299 187 bp, and the G+C content was 57.2%; two circular genomes of chromosome 1 and chromosome 2 were assembled; the size of chromosome 1 and chromosome 2 was 2 121 370 bp and 1 177 817 bp, respectively, and the G+C content of chromosome 1 and chromosome 2 was 57.2% and 57.3%, respectively. The sequences of Ery, BLS and VirB10 genes of the Brucella Rev.1 vaccine strain were bioinformatically compared and analyzed with their counterparts of 9 Brucella reference strains, and the sequence similarity among those genes were observed, ranging from 97.4% to 100%.