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| 血管瘤病变型J亚群禽白血病病毒E元件缺失突变体的构建 | |
| 引用本文： | 张贺楠,齐岩,史伟伟,梁艺瑜,刘洪波,张小桃,曹伟胜,廖明.血管瘤病变型J亚群禽白血病病毒E元件缺失突变体的构建[J].中国预防兽医学报,2010,32(2). |
| 作者姓名： | 张贺楠 齐岩 史伟伟 梁艺瑜 刘洪波 张小桃 曹伟胜 廖明 |
| 作者单位： | 1. 华南农业大学,兽医学院/农业部动物疫病防控重点开放实验室,广东,广州,510642;农业部动物疫病防控重点开放实验室,广东,广州,510642 2. 华南农业大学,兽医学院/农业部动物疫病防控重点开放实验室,广东,广州,510642;农业部动物疫病防控重点开放实验室,广东,广州,510642;中国牧工商(集团)总公司,北京,100070 3. 华南农业大学,兽医学院/农业部动物疫病防控重点开放实验室,广东,广州,510642 |
| 基金项目： | 国家自然科学基金，NSFC-广东联合基会，广东省自然科学基金，广东省科技计划项目 |
| 摘 要： | 为构建血管瘤病变型J亚群禽白血病病毒E元件缺失突变体病毒,本研究利用融合PCR方法将E元件缺失,获得含有缺失性突变体病毒前病毒全基因组的重组质粒pSCAU-HN-△E。将该重组质粒纯化后转染DF-1细胞,并连续传代,最终获得缺失病毒rSCAU-HN-△E。该毒株感染的DF-1细胞可被ALV-J特异性单克隆抗体JE9所识别,并且体外增殖性能稳定。该缺失性病毒的获得为E元件功能的研究奠定了基础。 |
| 关 键 词： | J亚群禽白血病病毒 血管瘤 E元件 感染性克隆 |
Construction of an infectious clone of subgroup J Avian leukosis virus with E element deletion |
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| ZHANG He-nan,QI Yan,SHI Wei-wei,LIANG Yi-yu,LIU Hong-bo,ZHANG Xiao-tao,CAO Wei-sheng,LIAO Ming.Construction of an infectious clone of subgroup J Avian leukosis virus with E element deletion[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(2). | |
| Authors: | ZHANG He-nan QI Yan SHI Wei-wei LIANG Yi-yu LIU Hong-bo ZHANG Xiao-tao CAO Wei-sheng LIAO Ming |
| Institution: | ZHANG He-nan1,2,QI Yan1,3,SHI Wei-wei1,LIANG Yi-yu1,LIU Hong-bo1,ZHANG Xiao-tao1,CAO Wei-sheng1,LIAO Ming1,2 (1. The South China Agricultural University,Key Laboratory of Animal Disease Control , Prevention,Ministry of Agriculture,College of Veterinary Medicine,Guangzhou 510642,China,2. Key Laboratory of Animal Disease Control , Prevention,3. China Animal Husb,ery Group,Beijing 100070,China) |
| Abstract: | The Subgroup J avian leukosis virus (ALV-J) swain SCAU-HN06 isolated from commercial layer hens with spontaneous hemangiomas has a complete "E" element in YUTR. In order to rescue the E element-deleted vires, we constructed the recombinant plasmid pSCAU-HN-△E that contained the whole ALV-J genome but with E element deleted. The plasmid was transfected into DF-1 cells and the rescued virus was identified by indirect fluorescence antibody test with ALV-J specific monoclonal antibody JE9. The recombinant virus was stable in cell culture during nine passages and had a titre up to 10~3.6TCID_(50)/0.2 mL. |
| Keywords: | subgroup J Avian leukosis virus Hemangioma Eelement infectious clone |
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