| 利用免疫共沉淀技术验证SET与eEF1A1在人肝细胞内的相互作用(摘要)(英文) |
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| 引用本文： | 杨亮,杨细飞,张毅,刘建军,李杰.利用免疫共沉淀技术验证SET与eEF1A1在人肝细胞内的相互作用(摘要)(英文)[J].农业科学与技术,2010(8):87-90. |
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| 作者姓名： | 杨亮 杨细飞 张毅 刘建军 李杰 |
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| 作者单位： | [1]华南理工大学生物科学与工程学院,广东广州510006 [2]深圳市疾病预防控制中心毒理研究室,广东深圳518020 |
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| 基金项目： | 国家自然科学基金资助项目（30972454）;深圳市科技计划重点项目（200801010）. |
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| 摘 要： | 目的]探讨SET与eEF1A1在人肝细胞内是否存在相互作用。方法]以人L-02肝细胞为材料,在非变性条件下提取总蛋白,将鼠抗人SET、兔抗人eEF1A1抗体分别加到总蛋白提取物中,用偶联了琼脂糖珠的ProteinA/G分离抗原抗体复合物,最后分别用兔抗人eEF1A1、鼠抗人SET抗体进行Western blotting检测。结果]在用SET抗体免疫共沉淀下来的蛋白复合物中检测到eEF1A1,同时在用eEF1A1抗体免疫共沉淀下来的蛋白复合物中也检测到SET。结论]SET与eEF1A1在人肝细胞内存在相互作用。
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| 关 键 词： | SET eEF1A1 免疫共沉淀 相互作用 |
Verification of the Interaction between SET and eEF1A1 in Human Liver Cells by Co-immunoprecipitation |
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| YANG Liang,YANG Xi-fei,ZHANG Yi,LIU Jian-jun,LI Jie.Verification of the Interaction between SET and eEF1A1 in Human Liver Cells by Co-immunoprecipitation[J].Agricultural Science ＆ Technology,2010(8):87-90. |
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| Authors: | YANG Liang YANG Xi-fei ZHANG Yi LIU Jian-jun LI Jie |
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| Institution: | 1. School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006; 2. Key Laboratory of Modern Toxicology of Shenzhen,Shenzhen Center for Disease Control and Prevention,Shenzhen 518020 |
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| Abstract: | Objective] The aim was to investigate the possible interaction between SET and eEF1A1 in human liver cells. Method] Firstly the total proteins of human L-02 liver cells were extracted under non-denaturing conditions; then,mouse anti-human SET and rabbit anti-human eEF1A1 antibodies were used to perform the co-immunoprecipitation respectively; subsequently,the immunoprecipitations was correspondingly detected with rabbit anti-human eEF1A1 and mouse anti-human SET antibodies by Western Blot. Result] EEF1A1 was detected in protein complex from the immunoprecipitations by using anti-SET antibody,and SET also was detected in immunoprecipitations by using anti-eEF1A1 antibody. Conclusion] The interaction between SET and eEF1A1 in human liver cells was confirmed. |
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| Keywords: | SET eEF1A1 Immunoprecipitation Interaction |
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