Lipopeptide Antibiotics Produced by the Engineered Strain Bacillus subtilis GEB3 and Detection of Its Bioactivity

MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics produced by GEB3 strain,a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene.The result showed GEB3 only produced lipopeptide antibiotic surfactin.The analysis by LC-MS demonstrated that GEB3 produced standard surfactin isoforms with side chain lengths of 13,14 and 15 carbon atoms.The bioactivity detection of surfactin indicated that the surfactin produced by GEB3 had inhibition effect on plant pathogens Rhizoctonia solani and Pyricularia oryzae.