| 比色法测定保鲜人参中人参总皂苷的含量 |
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| 引用本文： | 曲正义,赵景辉,刘宏群,姚春林,王英平.比色法测定保鲜人参中人参总皂苷的含量[J].人参研究,2012,24(1):13-14. |
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| 作者姓名： | 曲正义 赵景辉 刘宏群 姚春林 王英平 |
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| 作者单位： | 中国农业科学院特产研究所,吉林吉林,132109 |
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| 摘 要： | 目的采用索氏热回流提取保鲜人参中的人参总皂苷。方法香草醛一硫酸比色法测定保鲜人参中人参总皂苷的含量，检测波长544nm。结果索氏热回流提取可将保鲜人参中的人参总皂苷提取完全，在20-120μg（r=0．9995）范围内线性关系良好，平均回收率为107．86％，BSD=3．49％（n=5）。测得样品中总皂苷含量为3．91％，RSD=I．84％（11=3）。结论该方法操作简便，稳定性和重现性较好，可用于保鲜人参中人参总皂苷的含量测定。
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| 关 键 词： | 保鲜人参 索氏提取 人参总皂苷 比色法 含量测定 |
Determination of total ginsenoside content in fresh-keeping ginseng by colorimetry |
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| Authors: | QU Zheng-yi ZHA Jing-hui LIU Hong-qun YAO Chun-lin WANG Ying-ping |
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| Institution: | (Institute of Speehd Wild Economic Animal and Plato Sc iem： e,Jilin 132109) |
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| Abstract: | The fresh-keeping ginseng was extracted by soxhlet extraction with hot methanol reflux method, And then,The colorimetry method analysis was employed,The detected wavelength was 544 nm. Results indicated th;tt A good linear relationship was observed in the range of 20-120μg （r=0.9995）and averagerecovery rate was 107.86% with a relative standard deviation of 3.49%（n=5）. The content of total ginsenoside in the samples from Ji＇an city,Ji lin region was 3.91%,with 1.84% of RSD （n=3）. |
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| Keywords: | Fresh-keeping ginseng Soxhlet extraction Total ginsenoside Colorimetry Determination |
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