不同地理种源马尾松花粉几个生理特性的研究

本文以不同地理种源马尾松花粉为试验材料,进行了花粉营养元素、花粉萌发状况、花粉过氧化物酶同工酶及其性状变异的研究。结果表明,马尾松花粉含有N,P,K,Zn等15种营养元素。各地马尾松花粉营养元素含量存在差异,其中部分元素之间存在一定的比例关系。马尾松花粉管有着不同的类型。花粉管生长长度与置床时间呈线性关系,与蔗糖浓度呈三次抛物线关系。花粉多在5％蔗糖浓度的培养基上生长最好。马尾松花粉过氧化物酶同工酶分为A,B两区。酶带多分布在B区且活性强。其酶谱存在着不同谱型。花粉POD的酶带数目及相对活性可以做为其活力的一个生理指标。马尾松花粉营养元素含量、萌发特性及过氧化物酶同工酶绝大部分性状都没有地理变异规律。

Study on Several Physiological Traits of Proveaances Pinus massoniana Pollen

This paper, taking provenances P. massoniana Lamb, pollen as test materil, has studied several physiological traits of P. massoniana Lamb, pollen, such as neurion elements, germination and FOD. The result shows: 15 elements are measured in P. massoniana Lamb, pollen, such as N, P, K, Zn, Fe, etc. Parts of the elements are related; there are some rates among them and the quantity of each provenance element is different. The pollen tube has many types and the length of pollen tube is related to the germination time as linear and to the sucros concentration as cubic parabola. The growth of pollen is the best one in culture medium with 5% sucros concentration. The isozyme pattern of peroxdase (POD) of pollen has A and B regions. Many of the bands appear in B region. There are some types in the pollen's isozyme of POD. The bands and its relatively activity can be considered as a physiological index of the activity of pollen. In generally, the variation of neurion elements, germination and POD in P. massoniana Lamb, pollen are not geographic ones.