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| 副猪嗜血杆菌OMP P5间接ELISA抗体检测方法优化 | |
| 引用本文： | 臧莹安,黄彩梅,李婉雁,李淼,宋帅,李春玲.副猪嗜血杆菌OMP P5间接ELISA抗体检测方法优化[J].动物医学进展,2012(9):6-9. |
| 作者姓名： | 臧莹安 黄彩梅 李婉雁 李淼 宋帅 李春玲 |
| 作者单位： | 1. 仲恺农业工程学院动物科学系,广东广州510225 2. 广东省农业科学院兽医研究所,广东广州510640 3. 广东省兽医公共卫生公共实验室,广东广州510640 |
| 基金项目： | 广东省农业科技重大专项(2009A020101006);广州市农业科技重大专项(2009A1-E041);广东省兽医公共卫生公共实验室开放基金项目(GSKJ090202) |
| 摘 要： | 利用副猪嗜血杆菌(HPS)OMP P5基因表达并纯化复性后蛋白作为包被蛋白,通过对最佳加样作用时间的确定和最佳酶标二抗反应时间等条件的探索优化,建立针对副猪嗜血杆菌外膜蛋白P5(OMPP5)的间接ELISA抗体检测方法,通过对进行和未进行HPS免疫的健康猪血清的检测,证实了建立的间接ELISA方法具有敏感性高、特异性强、反应快速准确等特点,可用于HPS的快速诊断 |
| 关 键 词： | 副猪嗜血杆菌 外膜蛋白P5 酶联免疫吸附试验 |
Optimization of Indirect ELISA Using Haemophilus Parasuis OMP P5 for Antibody Detection |
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| ZANG Ying-an,HUANG Cai-mei,LI Wan-yan,LI Miao,SONG Shuai,LI Chun-ling.Optimization of Indirect ELISA Using Haemophilus Parasuis OMP P5 for Antibody Detection[J].Progress In Veterinary Medicine,2012(9):6-9. | |
| Authors: | ZANG Ying-an HUANG Cai-mei LI Wan-yan LI Miao SONG Shuai LI Chun-ling |
| Institution: | 2,3(1.Zhongkai University of Agriculture and Technology,Guangzhou,Guangdong,510225,China;2.Institute of Veterinary Medicine,Guangdong Academy of Agricutral Sciences,Guangzhou,Guangdong,510640,China;3.Guangdong Key Laboratory of Veterinary Public Health,Guangzhou,Guangdong,510640,China) |
| Abstract: | Using Haemophilus parasuis(HPS) OMP P5gene expressed and purified renaturation protein as coating protein,an indirect ELISA antibody detection method was established through optimizing every condition.The serafrom health pigs immunized without or with HPS were used to detected the antibody by the methed.The results confirmed that the indirect ELISA is highly sensitive,strongly specific,quick and accurate response,and can be used for rapid diagnosis of HPS infection. |
| Keywords: | Haemophilus parasuis OMP P5 ELISA |
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