福州动物园动物高水平耐庆大霉素肠球菌耐药表型检测

为了解福州动物园动物细菌耐药现状,选用肠球菌为指示菌,采用琼脂平板筛选法(ADSP法)筛选高水平耐庆大霉素肠球菌(HLGRE).根据美国临床实验室标准化协会(CLSI)推荐的微量肉汤稀释法测定耐药菌株对6种抗菌药物的耐药性,用PCR法对分离出的不同来源的高水平耐氨基糖苷类肠球菌进行菌种鉴定.从动物园圈养野生动物共分离出78株HLGRE,不同动物来源的分离率不同,其中草食动物HLGRE分离率最低,占55.56％;肉食动物HLGRE分离率最高,占84.38％.药敏试验结果显示,不同动物来源的HLGRE均对红霉素和土霉素的耐药率最高,分别为100％和96.1％,对氨苄西林和万古霉素的耐药率最低.

Detection of Resistant Phenotype of High-level Gentamicin-resistant Enterococcus from Fuzhou Zoo Animals

To explore the resistance of bacteria from Fuzhou animal zoo to antimicrobials,Enterococcus was used as directed bacteria and ADSP method was used to isolate high-level gentamicin-resistant Enterococus(HLGRE).A micro-broth-dilution method recommended by CLSI was used to test the susceptibility of Enterococcus to 6 kinds of antimicrobials.A PCR method was used to identify the HLGRE.The result showed that there were 78 strains of HLGRE isolated from a total 110 samples.The isolation ratio of HLGRE differed from different animals,which was lowest from herbivorous animals(55.56%) and highest form carnivorous animals(84.38%).The result of antimicrobial susceptibility test indicated that HLGRE from different animal sources showed highest resistant ratio to ERY and OTC,which was 100% and 96.1% respectively,and the resistant ratio was low to AMP and VAN.