| 绵羊肺腺瘤病毒囊膜蛋白在大鼠体内的表达 |
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| 引用本文： | 李靖,瓦西力,周艳喜,么宏强,于立新,敖威华,马学恩.绵羊肺腺瘤病毒囊膜蛋白在大鼠体内的表达[J].动物医学进展,2012,33(7):7-11. |
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| 作者姓名： | 李靖 瓦西力 周艳喜 么宏强 于立新 敖威华 马学恩 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院,呼和浩特,010018
2. 内蒙古出入境检验检疫局技术中心,呼和浩特,010020 |
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| 摘 要： | 为探讨绵羊肺腺瘤病毒囊膜蛋白(OPAV-Env)引起肺泡Ⅱ型上皮恶变的机理,将真核表达质粒pcDNA3.1-env用体内转染试剂经尾静脉转染到幼龄大鼠体内,用RT-PCR及免疫组化法检测绵羊肺腺瘤病毒囊膜蛋白在大鼠体内的表达,并采用SSCP法及基因克隆法结合序列分析检测H-ras基因是否发生突变.结果显示,绵羊肺腺瘤病...
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| 关 键 词： | 绵羊肺腺瘤病毒 ras基因 免疫组化 单链构象多态性 |
Expression of OPAV-Env Protein in Rats |
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| LI Jing
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Vasily Bodyak
,
ZHOU Yan-xi
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YAO Hong-qiang
,
YU Li-xin
,
AO Wei-hua
,
MA Xue-en.Expression of OPAV-Env Protein in Rats[J].Progress In Veterinary Medicine,2012,33(7):7-11. |
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| Authors: | LI Jing Vasily Bodyak ZHOU Yan-xi YAO Hong-qiang YU Li-xin AO Wei-hua MA Xue-en |
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| Institution: | 1(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia,010018,China;2.Inner Mongolia Enty-Exit Inspection and Quarantine Bureau,Huhhot,Inner Mongolia,010020,China) |
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| Abstract: | To explore the mechanism of alveolar typeⅡcell malignant transformation which was caused by the OPAV-env gene,the eukaryotic expression plasmid pcDNA3.1-env was transfected into young rats with vivo transfection reagent through tail vein injection method.Then the RT-PCR and immunohistochemistry were used to detect the expression of pcDNA3.1-env plasmid in rats.Mutations of ras gene was detected by using methods of SSCP,gene clone and sequence analysis.The results showed that the plasmid pcDNA3.1-env was expressed in lung tissue of rats.21days after transfection,the ras gene mutation was not discovered compared with normal rats. |
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| Keywords: | Ovine pulmonary adenomatosis virus ras gene immunohistochemistry single strand conformation polymorphism |
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