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白鹭源新城疫病毒的分离与鉴定
引用本文:陈安莉,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤,谢丽基,范睛.白鹭源新城疫病毒的分离与鉴定[J].动物医学进展,2012,33(7):119-121.
作者姓名:陈安莉  谢芝勋  刘加波  庞耀珊  邓显文  谢志勤  谢丽基  范睛
作者单位:1. 广西大学动物科学技术学院,广西南宁,530004
2. 广西兽医研究所广西畜禽疫苗新技术重点实验室,广西南宁,530001
基金项目:广西特聘专家专项经费,国家百千万人才工程入选专项费金
摘    要:从1只患病的白鹭的咽喉、泄殖腔棉拭子中分离到1株病毒,经血凝、血凝抑制试验和RT-PCR法鉴定为新城疫病毒。根据该毒株对鸡胚平均致死时间、鸡胚半数致死量、鸡胚半数感染量的测定和新城疫强弱毒鉴别的RT-PCR检测,表明该分离株为新城疫强毒株。

关 键 词:白鹭  新城疫病毒  分离  鉴定

Isolation and Identification of Newcastle Disease Virus from Egret
CHEN An-li , XIE Zhi-xun , LIU Jia-bo , PANG Yao-shan , DNG Xian-wen , XIE Zhi-qin , XIE Li-ji , FAN Qing.Isolation and Identification of Newcastle Disease Virus from Egret[J].Progress In Veterinary Medicine,2012,33(7):119-121.
Authors:CHEN An-li  XIE Zhi-xun  LIU Jia-bo  PANG Yao-shan  DNG Xian-wen  XIE Zhi-qin  XIE Li-ji  FAN Qing
Institution:1.College of Veterinary Medicine,Guangxi University,Nanning,Guangxi,530004,China;2.Guangxi Veterinary Research Institute,Guangxi Key Laboratory of Animal Vaccines and New Technology,Nanning,Guangxi,530001,China)
Abstract:A strain of virus was isolated from injured a Egret with throat and cloacae swabs to be Newcastle disease virus by hemagglutination test and hemagglutination inhibition test and PCR detection.The isolated virus was proved to be a strain of virulent virus by detecting its mean death time to chicken embryo,ELD50 to SPF chicken embryo,EID50 to SPF chicken embryo,and RT-PCR detection.
Keywords:Egret  Newcastle disease virus  isolation  identification
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