浓缩和纯化操作对口蹄疫A型弱毒病毒含量的影响

使用引进的美国Pall公司微滤、超滤和过滤系统对口蹄疫A型弱毒病毒的细胞培养液进行除细胞碎片纯化、病毒的浓缩和除菌等操作，取样进行病毒含量测定，以探索细胞培养病毒液除细胞碎片、浓缩和除菌后病毒含量的变化规律。结果显示，浓缩前液量少于21700mL时，经除细胞碎片微滤、超滤浓缩和除菌过滤后，毒价严重下降，原液、除碎片微滤液、10倍浓缩液、10倍浓缩除菌过滤液、滤出清液的LD50／0．2mL分别为10^7.5、10^6.5、10^7.5、10^7.25、10^2.0；浓缩前液量多于203200mL时，原液、除碎片微滤液、10倍浓缩液、10倍浓缩除菌过滤液、滤出清液的LD50／0．2mL分别为10^7.5、10^7.5、10^8.25、10^2.0，表明通过使用浓缩技术来提高A型弱毒细胞培养液的病毒含量是可行的。

Effects of Concentration and Purification on the Content of FMD Type A Attenuated Virus

Pall filtration,micro-filtration and ultra-filtration system were used to purify and concentrate foot and mouth disease(FMD)type A attenuated virus,which underwent a series of operations including removal of cellular scraps,concentration and filtration of bacteria.After that,the influential degree was tested.Results indicate that when the initial FMD virus fluid volume is lower than 21 700 mL,the LD50 of FMD virus decreases severely;the LD50 per 0.2 mL of initial fluid,fluid removed of cellular scraps,10 times concentrated fluid,10 times concentrated fluid free of bacterium and filtrate is 107.5,106.5,107.5,107.25,102.0 respectively;when the initial volume is above 203 200 mL,corresponding LD50 per 0.2 mL is 107.5,107.5,108.25,108.25,102.0 respectively.Conclusion can be made that concentrating and purifying FMD virus by Pall filtration,micro-filtration and ultra-filtration system is feasible.