黑龙江省主栽水稻品种抗稻瘟病基因的分子检测与分析

了解品种本身的抗性基因型对于水稻品种合理布局具有重要意义。为了明确稻瘟病抗性基因Pi-ta、Pi-b、Pik-m、Pi9、Pii、Pi-d3在黑龙江省水稻品种中的分布情况,选取34份主栽品种,利用这6个抗瘟基因的功能标记,对供试材料进行分子标记检测。结果表明,Pi9的分布频率最高,其次是Pi-ta和Pik-m,Pi-b和Pii抗性基因分布频率较低,Pi-d3的分布频率最低;供试的34份水稻材料中,被检测出最多含有5个抗性基因,而最少只含有1个抗性基因,含有2个抗性基因的品种所占比例最大,龙粳40和龙粳42不含有待检测基因。

Molecular Detection and Analysis of Rice Blast Resistance Genes in Main Rice Varieties in Heilongjiang Province

Understanding the resistance genotype of the variety itself is great significant for the rational layout of rice varieties. In order to clarify the distribution of rice blast resistance genes Pi-ta, Pi-b, Pik-m, Pi9, Pii and Pi-d3 in rice varieties in Heilongjiang Province, 34 main varieties were selected, and the functional markers of these 6 anti-blast genes were used to detect the molecular markers. Results showed that the distribution frequency of Pi9 was the highest, followed by Pi-ta and Pik-m, the frequency of Pi-b and Pii resistance genes was lower, and the distribution frequency of Pi-d3 was the lowest. Among the 34 rice materials tested, some varieties contained up to five resistance genes and some contained at least one resistance gene. The species with two resistance genes accounted for the largest proportion. Longjing 40 and Longjing 42 did not contain the gene to be detected.