甜椒疫病抗性遗传及相关基因分子标记研究

 以高抗疫病的甜椒育种材料‘N1345’与高感疫病辣椒材料‘N1308’为双亲，构建了6个联合世代，运用植物数量性状主基因 + 多基因联合分离分析方法对N1345疫病抗性进行遗传分析，并对其F2群体进行EST-SSR与SSR连锁标记分析。结果显示，N1345对疫病抗性由2对加性—显性—上位性主基因控制（B-1-1），两主基因加性效应、显性效应均相等，主基因遗传率在B1、B2和F2世代分别为63.43%、82.32%和83.46%。筛选到E73、E318等2个EST-SSR标记与疫病抗性病级均呈显著负相关，相关系数分别为﹣0.6296、﹣0.5492，差异水平均为极显著（P < 0.01），表明这2个标记与对疫病抗性基因紧密连锁。这2个标记在22份甜辣椒材料中分离一致，有6份材料抗、感表型与标记分离不一致。

Genetic and Molecular Marker Analysis of Resistance to Phythophtora capsici in Sweet Pepper

Using the model of the major gene plus polygene of quantitative traits,a joint analysis of six-generations was performed from cross between a sweet pepper N1345 with high resistance to phythophtora blight and a highly susceptible hot pepper N1308.F2 population was analysed with EST-SSR and SSR molecular markers.It was showed that the resistance to Phythophtora blight of N1345 was controlled by two major genes(B-1-1)with equal additive and dominant effects.And the major genes heritabilities of B1,B2,F2 were ...