| 三倍体葡萄种质创新及倍性快速鉴定 |
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| 引用本文： | 赵艳华,程和禾,吴雅琴,吴永杰,李玉生.三倍体葡萄种质创新及倍性快速鉴定[J].园艺学报,2011,38(6):1161-1166. |
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| 作者姓名： | 赵艳华 程和禾 吴雅琴 吴永杰 李玉生 |
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| 作者单位： | （河北省农林科学院昌黎果树研究所，河北昌黎066600） |
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| 基金项目： | 河北省农林科学院项目(A09020203) |
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| 摘 要： | 以二倍体葡萄品种玫瑰香、红地球、摩尔多瓦、无核奥迪亚及四倍体葡萄品种巨峰为亲本进行杂交授粉，对杂种进行胚培养，成株率最高可达30.6%。对杂种后代用流式细胞仪初选，确定30个杂交后代为三倍体植株。组合不同，获得三倍体植株的比例不同，目前获得的225个胚培养的葡萄杂种后代全部栽入大田。
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| 关 键 词： | 葡萄 胚培养 三倍体 倍性鉴定 |
| 收稿时间： | 2010-10-19 |
Germplas Development of Triploid Grapevine and Rapid Identification of Chromosomes Ploidy |
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| ZHAO Yan-hua,CHENG He-he,WU Ya-qin,WU Yong-jie,LI Yu-sheng.Germplas Development of Triploid Grapevine and Rapid Identification of Chromosomes Ploidy[J].Acta Horticulturae Sinica,2011,38(6):1161-1166. |
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| Authors: | ZHAO Yan-hua CHENG He-he WU Ya-qin WU Yong-jie LI Yu-sheng |
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| Institution: | （Changli Institute of Pomology，Hebei Academy of Agriculture and Forestry Sciences，Changli，Hebei 066600，China） |
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| Abstract: | Diploid grape'Muscat Hamburg','Red Globe','Moldova'and'Otilia seedless'were crossed with tetraploid parents'Kyoho'.The hybrid embryos were in vitro cultured and regenerated into plantlets.The regeneration rate of plantlets reaches to 30.6%.By using Flow Cytometry,thirty triploid cross progenies were obtained.The results showed that the percentages of triploid cross progenies was different when with the different cross combinations of parents.Recently 225 regenerated grape progenies were transplanted into fi... |
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| Keywords: | grape embryo culture triploid grape breeding identification of chromosome |
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