山核桃间接体细胞胚发生和植株再生

 以山核桃（Carya cathayensis Sarg.）自然授粉后10周的幼胚为外植体，对影响胚性愈伤组织和体胚发生的主导因子（基本培养基、植物生长调节物质等）进行了比较分析；对影响体胚萌发的脱水处理时间进行了比较；并采用石蜡切片法对幼胚脱分化产生胚性愈伤组织及体胚发生发育过程进行了组织细胞学观察。结果表明，幼胚胚轴和子叶接种在基本培养基1/2 MS上，胚性愈伤组织诱导率显著高于其它处理，达45.3%。接种于基本培养基DW中的幼胚体细胞胚诱导率显著高于其它培养基处理，达16.7%。显微镜下可观察到球形胚、心形胚、鱼雷胚和子叶胚。培养基添加1.0 mg · L-1 6-BA和0.01 mg · L-1 picloram（氨氯吡啶酸）组合时，胚性愈伤组织诱导率最高，为48.6%；而1.0 mg · L-1 6-BA与0.001 mg · L-1 picloram组合，体胚诱导率最高，为23.6%。体细胞胚发生方式属于间接体胚发生。用饱和Ca(NO3)2 · 4H2O对体胚进行脱水处理，脱水处理3 d后萌发率为39.03%，显著高于对照及其余处理。将脱水处理后的体胚接种至WPM基本培养基中，光照条件下培养，10周后可长成具3 ~ 4片真叶的完整植株。

Indirect Somatic Embryogenesis and Plant Regeneration of Carya cathayensis

Immature embryos of 10 weeks after pollination of Carya cathayensis were selected as explants.The factors which affect embryogenic calli formation and somatic embryogenesis including basal medium,plant growth regulators(PGRs)and desiccation length of somatic embryos were tested.Cytohistological observations of anatomical structure change during embryogenic callus formation and somatic embryos production were also made.Embryogenic callus percentage was significantly higher onthe medium 1/2 MS than other trea...