GFP标记的球孢白僵菌在玉米中的定殖

为明确球孢白僵菌在玉米中的定殖分布规律以及不同施用方式和处理浓度对玉米生长发育的影响，本文利用绿色荧光蛋白（GFP）标记的球孢白僵菌，在激光扫描共聚焦显微镜下观测了球孢白僵菌在玉米根部、茎部和叶部的定殖情况，同时检测了不同接种方式在球孢白僵菌不同处理浓度下对玉米生长指标的影响。结果表明，球孢白僵菌在玉米不同组织部位上的定殖有着明显的差异，各球孢白僵菌接种处理中，均以叶部定殖的白僵菌孢子数最多，而茎部均未观测到孢子定殖；玉米根部仅在浸种和灌根处理中可见少量孢子。球孢白僵菌不同接种方式对其在玉米中定殖率的影响差异较大，灌根处理定殖率最高，为76.7%；其次为浸种处理，为73.3%；茎部注射处理及叶面喷施处理定殖率较低，分别为43.3%和36.7%。研究表明，不同施用方式及接种浓度球孢白僵菌孢悬液对玉米生长指标有一定的影响，球孢白僵菌可通过不同接种方式在玉米植株中定殖并扩散，对玉米的生长发育有一定的促进作用，其中以1×10⁶~1×10⁷孢子/mL球孢白僵菌孢悬液浸种或灌根，对玉米苗的促进生长作用最明显。

Colonization of GFP-tagged Beauveria bassiana in Maize

To understand the colonization and distribution of Beauveria bassiana in maize and the effects of different application methods and concentrations on the growth and development of maize, the colonization of B. bassiana in the roots, stems and leaves of maize was observed by confocal laser scanning microscope using green fluorescent protein (GFP)-labeled B. bassiana. Our results showed obvious differences in the colonization of B. bassiana in different tissues of maize. The number of spores colonized in leaves was the highest, while no spores were observed in the stem area; a small number of spores was observed in the roots of maize that were treated only by seed soaking and root-irrigation treatments. The inoculation methods showed a great influence on the colonization rate of B. bassiana in maize. The highest colonization rate of 76.7% occurred in root-irrigation treatment, followed by seed soaking treatment (73.3%), while colonization rates were lower in foliar spraying and stem injection treatment (43.3% and 36.7%, respectively). In conclusion, different inoculation methods and dosages of B. bassiana suspension have certain effects on growth of maize, and B. bassiana can colonize and spread in maize and promote the growth and development of maize, with seed soaking and root irrigation at 1×10⁶-1×10⁷ spores/mL being the optimal methods.