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| 棉花黄萎病菌拮抗细菌TYG-2抑菌蛋白的分离纯化 | |
| 引用本文： | 孙瑶. 棉花黄萎病菌拮抗细菌TYG-2抑菌蛋白的分离纯化[J]. 山西农业大学学报(自然科学版), 2011, 31(5): 400-407 |
| 作者姓名： | 孙瑶 |
| 作者单位： | 太原工业学院环境与安全工程系,山西太原,030008 |
| 摘 要： | 拮抗细菌TYG-2是从根际土壤中分离得到的一株芽孢杆菌,其菌株发酵液产生的抑菌物质对棉花黄萎菌具有较强的抑制作用。为了得到它的抑菌活性组分,将TYG-2菌株去菌体发酵液经硫酸铵沉淀,得到的沉淀物脱盐后经DEAE-Sepharose Fast Flow分离得到4个组分,经抑菌活性测定得到一个抑菌活性组分,再通过Sephacry S-100凝胶过滤分离得到两个组分P1和P2,经检测表明P1组分对V.dahliae的菌丝生长具有抑制作用,为有效抑菌活性组分。经SDS-PAGE电泳纯化为单一蛋白带,分子量约为30kDa。 |
| 关 键 词： | 棉花黄萎菌 拮抗细菌 抑菌蛋白 分离纯化 |
Purification of the Antifungal Protein from the Antagonistic Bacterium TYG-2 against Verticillium dahlae |
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| SUN Yao. Purification of the Antifungal Protein from the Antagonistic Bacterium TYG-2 against Verticillium dahlae[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2011, 31(5): 400-407 | |
| Authors: | SUN Yao |
| Affiliation: | SUN Yao(Department of Environment and Safety Engineering,Taiyuan Institute of Technology,Taiyuan Shanxi 030008,China) |
| Abstract: | Bacillus TYG-2was isolated from the cotton soil,whose fermentation liquid had antifungal substance against Verticillium dahilae Kleb.In order to get the antifungal substance,precipitates of the fermentation liquid from strain TYG-2 were collected by saturation ammonium sulfate.When the antifungal substance was purified by chromatography after desalting,four peaks were obtained from DEAE-Sepharose Fast the Flow,the fourth peak showed antifungal activity.After purified through Sephacry S-100,one of the two pe... |
| Keywords: | Verticillium dahlae Antagonistic bacterium Antifungal protein Purification |
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