巧克力中香兰素的快速分离柱高效液相色谱法测定

目的]探讨快速分离柱高效液相色谱法测定巧克力中的香兰素的效果。方法]采用快速分离柱高效液相色谱法测定巧克力中的香兰素。结果]选用40％甲醇40ml超声振荡10min提取巧克力中的香兰素，然后用固相萃取预分离，以ZORBAX Stable Bound（4．6×50mm，1．8μm）C18为固定相，甲醇-水（35：65）为流动相分离，流速为10ml／min，用紫外二极管矩阵检测器检测。在该色谱条件下，香兰素在1．0min内可达到基线分离。样品的标准回收率为98％-103％，相对标准偏差为0．46％一0．58％。和常规色谱相比可缩短85％以上的分离时间。结论]该方法简便快速，结果可靠，在一定程度上为巧克力中香兰素的快速测定提供了方法。

Determination of Vanillin in Chocolate by Rapid Column High Performance Liquid Chromatography

Objective]The aim was to discuss the effect of using rapid column high performance liquid chromatography to determine vanillin in chocolate. Method]The rapid column high performance liquid chromatography was used to determine vanillin in chocolate. Result]40 ml 40% methanol were selected to extract vanillin from chocolate by ultrasonic for 10 min, then solid phrase extraction was applied for pre-separation. The vanillin was separated with ZORBAX Stable Bound (4.6×50 mm, 1.8 μm) C18 as stationary phase and methanol-water (35∶65) as mobile phase at flow-rate of 10 ml/min, and the detector was UV photodiode array detector. Under the chromatographic conditions, vanillin could reach baseline separation in 1.0 min. The sample standard recoveries were 98%-103% and the relative standard deviations were 0.46%-0.58%. The separation time could be shortened above 85% compared with conventional chromatograph. Conclusion]The method was convenient and rapid, and the result was reliable, which provided method for rapid determination of vanillin in chocolate to some extent.