Microsatellite marker isolation and cultured strain identification in <Emphasis Type="Italic">Carassius auratus gibelio</Emphasis> |
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Authors: | Wei Guo Jian-Fang Gui |
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Institution: | (1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China;(3) Institution of Biological and Medical Technology, Fuzhou University, Fuzhou, 350001, China |
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Abstract: | Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study
a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from
the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design
locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel
carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and
length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different
microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected
in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different
cultured strains for selective breeding and aquaculture practice of gibel carp. |
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Keywords: | Gibel carp Microsatellite Gynogenesis Polyploidy Molecular marker |
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