| 三步层析法分离毕赤酵母工程菌中表达的新疆家蚕抗菌肽 |
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| 引用本文： | 窦君,郑树涛,王娉,张富春.三步层析法分离毕赤酵母工程菌中表达的新疆家蚕抗菌肽[J].新疆农业科学,2007,44(5):710-714. |
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| 作者姓名： | 窦君 郑树涛 王娉 张富春 |
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| 作者单位： | 新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐,830046 |
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| 基金项目： | 新疆高技术研究发展计划;新疆高等学校创新研究群体基金 |
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| 摘 要： | 通过离子交换层析、疏水作用层析和分子排阻层析等三步层析的方法,分离纯化了毕赤酵母工程菌所表达的新疆家蚕抗菌肽.结果表明,三步层析后,SDS电泳显示单一条带,分子量约为7.0 kDa;所纯化的新疆家蚕抗菌肽具有较强的抗菌活性.该纯化工艺的建立,为新疆家蚕抗菌肽在饲料添加剂、农用抗生素等方面的广泛应用奠定基础.
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| 关 键 词： | 新疆家蚕抗菌肽 离子交换层析 疏水作用层析 分子排阻层析 纯化 |
| 文章编号： | 1001-4330（2007）05-0710-05 |
| 收稿时间： | 2007-06-25 |
| 修稿时间： | 2007-07-25 |
Isolation and Purification of Cecropin-XJ Expressed in Pichia Pastoris by Three Chromatographic Methods |
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| DOU Jun,ZHENG Shu-tao,WANG Ping,ZHANG Fu-chun.Isolation and Purification of Cecropin-XJ Expressed in Pichia Pastoris by Three Chromatographic Methods[J].Xinjiang Agricultural Sciences,2007,44(5):710-714. |
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| Authors: | DOU Jun ZHENG Shu-tao WANG Ping ZHANG Fu-chun |
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| Institution: | Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China |
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| Abstract: | Cecropin-XJ expressed in Pichia pastoris was purified through ion exchange chromatobraphy(IEC),hydrophobic interaction chromatography(HIC) and size exclusion chromatography(SEC).The results showed that there was a single band and its molecular weight was 7.0 kDa.The purified Cecropin-XJ was more active for the antibacterial effects.This purification strategy was established,which will provide a basis for potential application of antibacterial peptide in feed additive and antibiotics used for agriculture,and so on. |
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| Keywords: | Cecropin-XJ ion exchange chromatography hydrophobic interaction chromatography size exclusion chromatography purification |
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