畜禽沙门氏菌药敏性研究及两种测定方法比较

采用Kirby-Bauer纸片扩散法(以下简称K-B法)和梅里埃公司的ATB Expression自动细菌鉴定及药敏分析仪(以下简称ATB法),测定了36株畜禽源沙门氏菌对16种抗生素的敏感性。结果表明:ATB法和K-B法对阿莫西林/克拉维酸、亚胺培南和复方新诺明的测定结果有显著性差异(P<0.05),对其他13种药物的测定结果没有显著性差异;全部菌株耐1种以上的抗生素,58%的菌株耐2种以上的抗生素。得出以下结论:在畜禽源沙门氏菌药敏性研究中,ATB法还不能完全替代K-B法;畜禽源沙门氏菌分离菌株不仅耐药性较强,而且耐药谱也在不断扩大。

Study on Susceptibility of Salmonella from Livestock and Poultry to Drugs and Comparison of Two Testing Methods

The susceptibilities of 36 Salmonella strains isolated from livestock and poultry products to 16 antibiotics were tested by using Kirby-Bauer slip-diffusion method(K-B method) and the method of BioMerieux ATB Expression automatic identification(ATB method).The statistical results showed that there were significant differences in the testing results of Salmonella susceptibility to Amoxicillin/Clavulanic acid,Imipenem and Sulfamethoxazole-trimethoprim between ATB method and K-B method(P < 0.05),but no significant differences in the testing results were found between the two methods for other 13 antibiotics.All the isolated strains were resistant to one or more antibiotics,and 58% of the strains were resistant to two or more antibiotics.In summary,ATB method still could not completely replace K-B method in the studies on the drug susceptibility of Salmonella strains derived from livestock and poultry.The isolated strains had stronger drug resistance,and their drug-resistant spectrum also continued to expand.