约氏乳杆菌对大鼠生长性能、血液指标及脏器指数的影响

本试验旨在研究约氏乳杆菌对大鼠生长性能、血液指标及脏器指数的影响。试验选取SD大鼠80只,雌雄各半。大鼠按照体重相近的原则随机分为4个剂量组,即阴性对照组、试验Ⅰ(5 000 mg/kg体重)、试验Ⅱ(1 000 mg/kg体重)和试验Ⅲ组(200 mg/kg体重)。试验期为30 d。结果表明:试验剂量为200~1 000 mg/kg体重时,约氏乳杆菌对大鼠体增重、总采食量、饲料转化率、血液学指标和病理学指标的影响无显著性差异(P>0.05);试验剂量为5 000 mg/kg体重时,谷草转氨酶、血糖、总胆固醇差异不显著(P>0.05);与其他剂量组相比,大鼠的体增重和总采食量显著降低(P<0.05),但饲料转化率无显著差异(P>0.05);谷丙转氨酶水平显著降低(P<0.05),白蛋白和总蛋白显著增高(P<0.05);雌性大鼠尿素氮和雄性大鼠肌酐显著增高(P<0.05)。综合分析,约氏乳杆菌剂量低于1 000 mg/kg体重时,对大鼠平均日增重、血液指标及脏器指数无影响;约氏乳杆菌剂量高于5 000 mg/kg体重时,对大鼠脏器指数有影响。

Effects of Lactobacillus johnsonii on Growth,Blood Indices and Organ Indices in SD Rats

This experiment was conducted to explore the effects of Lactobacillus johnsonii on average daily gain, blood indices and organ indices in rats. Eighty Sprague-Dawley rats, half of male and female, were randomly allotted into 4 groups in accordance with the principle of similar weight: Negative control group, group Ⅰ(5 000 mg/kg body weight), group Ⅱ(1 000 mg/kg body weight) and group Ⅲ(200 mg/kg body weight). Each group had 20 rats, half of male and female. The experiment lasted for 30 days. Lactobacillus johnsonii was added to drinking water and the dosage was calculated as 20% of the weight of the rats. The results showed as follows: there was no significant difference(P>0.05) on BWG, total feed intake, FCR, blood indices and organ indices of rats when the dosage was 200 mg/kg and 1 000 mg/kg body weight. When the dosage was 5 000 mg/kg body weight, there was no significant difference(P>0.05) on aspartate transaminase, blood glucose and total cholesterol. Compared with other groups, the alanine aminotransferase were significantly decreased(P <0.05); serum albumin and total protein were significantly increased(P <0.05); while blood urea nitrogen level of female and creatinine level of male rat were significantly increased(P<0.05). It is concluded that when the dosage ofLactobacillus johnsonii was lower than 1 000 mg/kg body weight, it had no effect to rats on average daily gain, blood indices and organ indices; when the dosage of Lactobacillus johnsonii was up to 5 000 mg/kg body weight, it had an effect on the organ index of rats.