苏姜猪A-FABP基因遗传多态性及其与肉质性状的关联分析

研究采用PCR-RFLP技术分析苏姜猪、姜曲海猪、长白猪群体A-FABP基因遗传多态性,并用统计学方法分析基因座多态与肉质性状的相关性。结果表明:3个猪种A-FABP基因内含子1均具有Bsm I酶切多态性,分别检测到3种基因型(AA、AB和BB),χ~2适合性检验显示,3个猪种A-FABP基因型分布处于Hardy-Weinberg平衡(P0.05)。多态信息含量(PIC)分析显示,3个品种猪均为中度多态;基因型与肉质性状关联分析显示,不同基因型肌内脂肪含量、大理石纹差异显著,大理石纹AA、AB型显著高于BB型(P0.05),肌内脂肪含量AA显著高于AB、BB型(P0.05)。结论显示,苏姜猪、姜曲海猪、长白猪A-FABP基因intron 1多态性对肉质性状影响显著。

Genetic Polymorphism of A-FABP Gene and Correlation Analysis with Meat Quality Traits in Sujiang Swine

Aesearch by PCR-RFLP technical analyzed Sujiang swine, Jingquhai swine and landrace groups' A-FABP genetic polymorphisms, and analyzed the correlation between loci polymorphism and meat quality traits. Results showed that the three swine-breeds' A- FABP gene intron 1 have Bsm I enzyme polymorphisms, three genotypes were detected(AA, AB, and BB). The Chi-square test results showed that three swine-breeds' A-FABP genotype distribution in Hardy-Weinberg equilibrium(P >0.05).Polymorphism information content(PIC), which were moderately polymorphic in three varieties of swines.According to the association analysis of different genotypes of marbling and intramuscular fat content(IMF), significant difference was found in marbling, genotype AA, AB was significantly higher than BB(P<0.05), intramuscular fat content genotype AA was significantly higher than AB, BB(P<0.05). Conclusion showed that Sujiang swine, Jiangquhai swine and landrace groups' A-FABP gene intron 1 polymorphism had significant effects on meat quality traits.