Natural occurrence of Pseudomonas aeruginosa,a dominant cultivable diazotrophic endophytic bacterium colonizing Pennisetum glaucum (L.) R. Br. |
| |
| Institution: | 1. Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic;2. Department of Civil Engineering, Monash University in Melbourne, 23 College Walk Clayton, Vic 3800, Australia;3. Department of Geography, Ghent University, Ghent, Belgium;4. Research Group Climate Change and Security, Institute of Geography, University of Hamburg, Hamburg, Germany;1. Universidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal;2. I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal;3. CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central da Gandra 1317, 4585-116 Gandra, Portugal;4. Programa de Pós-graduação em Ciências do Solo, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, Brazil;1. Departamento de Ingeniería Agrícola, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de las Villas, Carretera a Camajuaní km 5 ½, Santa Clara, VC, Cuba;2. Centro de Mecanización Agropecuaria, Facultad de Ciencias Técnicas, Universidad Agraria de la Habana, Carretera a Tapaste, San José, Mayabeque, Cuba;3. Centro de Investigación en Métodos Computacionales y Numéricos en la Ingeniería, Facultad de Construcciones, Universidad Central ‘‘Marta Abreu’’ de las Villas, Carretera a Camajuaní km 5 ½, Santa Clara, VC, Cuba |
| |
| Abstract: | Endophytic microbial communities can have strong influence on the growth of their host plants. The present study reports a diazotrophic endophytic bacterial species that colonizes predominantly Pennisetum glaucum (pearl millet) and remains stabilized throughout the latter's various growth stages under field conditions. Qualitative and quantitative changes in the endophytic bacterial population structure were examined during the plant growth period at regular intervals of 7 days up to harvesting. DNA fingerprinting (ERIC-PCR) was used as a biomarker to track the identity of various isolates obtained at different time intervals. Identification of representative bacterial species corresponding to different ERIC types was made on the basis of 16S rRNA gene sequence analysis. Based on the colony forming unit (cfu) count of bacterial isolates observed at various growth stages, Pseudomonas aeruginosa PM389 was found to be the dominant diazotrophic species among the cultivable endophytes colonizing pearl millet. The population of P. aeruginosa was detected in the host 21 days after sowing (DAS), indicating its entry in plant roots from soil and suggesting its non-vertical transfer in pearl millet. Moreover, an upward migration of this dominant diazotrophic bacterial species to shoots was observed with the plant growth. Further evaluation of P. aeruginosa PM389 revealed its various plant growth promoting properties viz. nitrogen fixation, mineral phosphate solubilization, siderophore production and antagonistic properties. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
