| 免疫亲合色谱及在农药残留分析中的应用 |
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| 引用本文： | 邵秀金,刘曙照,冯大和,钱传范.免疫亲合色谱及在农药残留分析中的应用[J].农药学学报,2003,5(4):9-14. |
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| 作者姓名： | 邵秀金 刘曙照 冯大和 钱传范 |
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| 作者单位： | 1.扬州大学 植物保护系,江苏 杨州 225009 |
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| 基金项目： | 国家自然科学基金29977028，国家自然科学基金20177019资助项目. |
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| 摘 要： | 免疫亲合色谱可用于复杂样本中微量目标分析物的选择性分离富集。综述了免疫亲合色谱的基本原理、方法的建立过程(包括抗体的制备、固相基质的筛选、抗体与基质的偶联、免疫亲合色谱条件的优化)、免疫亲合色谱在农药残留分析中的应用现状及发展趋势。
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| 关 键 词： | 免疫亲合色谱 农药残留 |
| 文章编号： | 1008-7303(2003)04-0009-06 |
Immunoaffinity Chromatography and its Application in Pesticide Residue Analysis |
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| SHAO Xiu-jin,LIU Shu-zhao,FENG Da-he and QIAN Chuan-fan.Immunoaffinity Chromatography and its Application in Pesticide Residue Analysis[J].Chinese Journal of Pesticide Science,2003,5(4):9-14. |
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| Authors: | SHAO Xiu-jin LIU Shu-zhao FENG Da-he and QIAN Chuan-fan |
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| Institution: | 1.Department of Plant Protection2.Yangzhou University, Yangzhou 225009, China3.Center of Physical and Chemical Analysis, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | Immunoaffinity chromatography (IAC) could be used for the separation and enrichment of trace target small molecular compounds such as pesticides in complicated samples with high specificity. The review is on the principle of IAC, the developmental procedure of IAC(including the preparation of antibody, the (selection) of matrix, the coupling of antibody to matrix, and the optimization of IAC condition), the (application) of IAC in pesticide residue analysis and the trend of this technology. |
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| Keywords: | immunoaffinity chromatography pesticide residue |
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