| 转反义PLDγ基因小麦的获得及其抗白粉病的初步鉴定 |
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| 引用本文： | 刘芳,吕维涛,崔德才,赵檀方.转反义PLDγ基因小麦的获得及其抗白粉病的初步鉴定[J].山东农业科学,2006(2):24-26,34. |
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| 作者姓名： | 刘芳 吕维涛 崔德才 赵檀方 |
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| 作者单位： | 1. 山东农业大学农学院,山东,泰安,271018
2. 山东农业大学生命科学学院,山东,泰安,271018 |
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| 基金项目： | 山东省小麦良种产业化项目 |
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| 摘 要： | 利用穗茎注射法将反义PLDγ基因导入烟农15和农大1521两个小麦品种中,旨在获得抗病性增强的转基因植株。结果表明:T3代经PCR及PCR-Southern杂交鉴定,目的基因已经整合到小麦的基因组中,并能在小麦基因组内稳定遗传;烟农15和农大1521的6个转基因株系的抗白粉病的能力明显增强。
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| 关 键 词： | 小麦 反义PLDγ基因 白粉病 |
| 文章编号： | 1001-4942（2006）02-0024-04 |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2005-12-26 |
Obtained transgenic wheat induced by anti-PLDγ gene and its primary identification of powdery mildew resistance |
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| LIU Fang,Lü Wei-tao,CUI De-cai,ZHAO Tan-fang.Obtained transgenic wheat induced by anti-PLDγ gene and its primary identification of powdery mildew resistance[J].Shandong Agricultural Sciences,2006(2):24-26,34. |
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| Authors: | LIU Fang Lü Wei-tao CUI De-cai ZHAO Tan-fang |
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| Abstract: | In order to obtain transgenic plants with enhanced powdery mildew resistance,antisense phospholipase Dγ(PLDγ) gene was introduced into two wheat cultivars Yannong 15 and Nongda 1521 by earstem injecting.By PCR and PCR-Southern analysis,anti-PLDγ gene had been integrated into wheat genomes of T_3 generation with genetic stability.Powdery mildew resistance test showed that the ability of 6 transgenic lines were enhanced. |
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| Keywords: | Wheat Antisense phospholipase Dγ gene Powdery mildew |
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