酿酒酵母菌关键酶基因剔除对谷胱甘肽合成的影响

目的]通过基因工程手段改良酿酒酵母菌以提高谷胱甘肽的产量。方法]利用基因重组技术分别构建CYS3基因剔除菌和CYS4基因剔除菌,对比了野生菌与突变酵母菌的生长曲线,并定量检测2者中谷胱甘肽和氨基酸的含量,得出CYS3基因和CYS4基因剔除对谷胱甘肽和氨基酸代谢的影响程度。结果]与野生菌株相比,CYS3基因剔除菌中谷胱甘肽含量降低了26.1%,CYS4基因剔除菌中谷胱甘肽含量提高了6.0%。结论]综合CYS3和CYS4基因剔除和其他在谷胱甘肽代谢途径中关键酶基因剔除试验结果所得数据,可以建立酿酒酵母谷胱甘肽代谢网络系统生物学模型。

Effects of Key Enzymatic Gene Deletion in Saccharomyces cerevisiae on Glutathione Synthesis

Objective] The research aimed to modified Saccharomyces cerevisiae to enhance the production of glutathione by genetic engineering tools.Method] CYS3 and CYS4 deletion strains were constructed by homologous recombination method.The glutathione metabolism and amino acids metabolism production were examined and compared them to relative production of wild strain of Saccharomyces cerevisiae.Then the effects of CYS3 and CYS4 deletion on glutathione metabolism and amino acids metabolism were figured out.The purpose was to know the effects of CYS3 and CYS4 on glutathione and amino acids synthesis by CYS3 and CYS4 deletion in Saccharomyces cerevisiae.Result] Compared with the wild strain,the production of glutathione in the CYS3 deletion strain reduced by 26.1%,the production of glutathione in the CYS4 deletion strain increased by 6.0%.Conclusion] Based on these data along with other glutathione synthesis related genes data,the network of Saccharomyces cerevisiae glutathione metabolism systems biology model could be established.