炎消热清的镇痛作用及其机理研究

为了研究炎消热清(AEE)的镇痛作用及其可能的机制,采用热板法和扭体法考察AEE的镇痛效果,并观察热刺激下二乙基二硫代氨基甲酸钠(DDC)、氟哌啶醇(Hal)、L-色氨酸(L-Trp)、赛庚啶(CYP)等药物对AEE镇痛作用的影响,用紫外分光光度法测定了热刺激所致疼痛小鼠全脑中的前列腺素E2(PGE2)含量。结果表明,AEE可明显减少醋酸扭体试验中小鼠的扭体次数,降低热刺激小鼠疼痛反应;可显著降低热刺激所致疼痛小鼠全脑中PGE2的含量和二乙基二硫代氨基甲酸钠对痛觉的敏感度,与氟哌啶醇之间存在明显的协同效应,与L-色氨酸和赛庚啶之间拮抗效应显著。说明AEE具有明显的镇痛作用,镇痛机制可能与其降低小鼠全脑中的PGE2含量以及增加去甲肾上腺素(NE)的含量、阻断多巴胺(DA)受体有关。

Analgesic roles of aspirin eugenol ester and its mechanisms

The analgesic roles of aspirin eugenol ester(AEE) were investigated by using Writhing test and Hot plate test,while the influence of some drugs,including Diethyldithiocarbamate(DDC),haloperidol(Hal),L-tryptophan(L-Trp) and cyproheptadine(Cyp),on the analgesic effects under heat stimulation were examined.Also,the contents of PGE2 in the whole brain of mice were measured by ultraviolet spectrophotometry.The result showed that both the writhing induced by acetic acid and the paining by thermal stimulation in mice were significantly eased by AEE;the contents of PGE2 in the whole brain of mice were decreased;the algesa induced by DDC was inhibited;a combined effect between Hal and AEE existed,while L-Trp/CYP had significant antagonism to AEE.It suggested that AEE has an obvious analgesic effect which may be related to decreasing the production of the PGE2 in the whole brain of rats,raising the contents of norepinephrine(NE) and blocking-up the dopamine(DA) receptor.