燕山板栗种质资源遗传多样性的RAPD分析

为研究燕山板栗的遗传多样性,采用随机扩增多态DNA(randomamplifiedpolymorphicDNA,RAPD)技术对36份燕山板栗种质进行了分析。分析了燕山板栗的遗传丰富度,并对包括36个燕山板栗品种和8份外来板栗品种在内的44份板栗种质进行聚类分析。结果表明,RAPD能有效地区分品种间的差异,用16个随机引物经PCR扩增共得到132个片段,其中有83个多态性片段,占62.9%;不同遗传位点之间遗传多样度最大可达0.444,最小值为0.096,平均多样度为0.187;UPGMA法聚类,将44份板栗种质聚成4个大的类群,36份燕山板栗可分为3个大的类群,外来种质聚为一类。燕山板栗明显不同于外来品种。在RAPD图谱中,找到了19个品种(类型)的特异性标记和标记组合,可作为品种(类型)分子鉴别的依据。

Analysis of genetic diversity of Yanshan chestnut by RAPD

Yanshan area is an important chestnut producing and export base in china. In order to explore the genetic diversity of the local chestnut germplsm, 36 local cultivars and 8 cultivars collected from the other producing areas were used as materials for studying their diversity by RAPD (random amplified polymorphic DNA markers). Clustering was executed among them. The results showed that all the cultivars could be identified by RAPD method. 16 random primers produced 132 DNA fragments and the polymorphic fragments amounted to 62.9%. The genetic diversity among 83 loci ranged from 0.096 to 0.444 and average genetic diversity was 0.187. The dendrogram generated by UPGMA clustered all the 44 cultivars into 4 groups. There was obviously difference between Yanshan chestnut cultivars and exotic cultivars. In addition, we found some specific markers or combination of markers for 19 cultivars which could be used to identify these cultivars.