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| 肉食兽细小病毒通用PCR诊断技术的建立 | |
| 引用本文： | 刘维全,范泉水,江禹,夏咸柱,黄耕,王蕾,王吉贵,房金波.肉食兽细小病毒通用PCR诊断技术的建立[J].中国兽医学报,2001,21(3):249-251. |
| 作者姓名： | 刘维全 范泉水 江禹 夏咸柱 黄耕 王蕾 王吉贵 房金波 |
| 作者单位： | 1. 解放军军需大学军事兽医研究所, 2. 中国农业大学 生物学院，北京 100094 3. 东北师范大学生物系, |
| 摘 要： | 根据肉食兽细小病毒核苷酸序列高度同源的特点，设计合成了1对引物，以犬细小病毒、貂肠炎病毒和猫泛和白细胞减少症病细胞培养物为DNA模板，进行PCR特异性片段扩增，扩增片段大小为0．6kb。结果表明，扩增产物与设计的2个引物之间的序列大小一致。通过通用性、特异性与敏感性试验及对临床检样品检测，证明本法对肉食兽细小病毒通用。且具有快速、特异和高度敏感的特点。 |
| 关 键 词： | 肉食兽 细小病毒 PCR 诊断技术 |
| 文章编号： | 1005-4545(2001)03-0249-03 |
| 修稿时间： | 2001年2月22日 |
Establishment of a Commonly used PCR Technique for Detection of Carnivore Parvoviruses |
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| LIU Wei quan ,FAN Quan shui ,JIANG Yu ,XIA Xian zhu ,HUANG Geng ,WANG Ji gui ,FANG Jin bo ,WANG Lei.Establishment of a Commonly used PCR Technique for Detection of Carnivore Parvoviruses[J].Chinese Journal of Veterinary Science,2001,21(3):249-251. | |
| Authors: | LIU Wei quan FAN Quan shui JIANG Yu XIA Xian zhu HUANG Geng WANG Ji gui FANG Jin bo WANG Lei |
| Institution: | LIU Wei quan 1,FAN Quan shui 1,JIANG Yu 1,XIA Xian zhu 1,HUANG Geng 1,WANG Ji gui 2,FANG Jin bo 3,WANG Lei 1 |
| Abstract: | Based on the property that the nucleic acid sequence of carnivore parvoviruses was high homologous each other, two primers were designed by means of computer and synthesized. And PCR was established for detection of canine parvovirus, feline panleukopenia virus and mink enteritis virus which was from the isolates of cell culture respectively. The diagnostic technique for these viruses above was proved to be very specific, sensitive and simple. |
| Keywords: | Carnivore parvovirus PCR detection |
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