白藜芦醇对绵羊卵母细胞体外受精的影响

本试验旨在研究不同浓度（0、0.5、2.0、5.0 μmol/L）白藜芦醇（resveratrol，RES）对绵羊卵母细胞体外受精（in vitro fertilization，IVF）、卵母细胞抗氧化能力及卵丘细胞分泌类固醇激素的影响。绵羊卵母细胞在含不同浓度RES的体外成熟（in vitro maturation，IVM）液中培养24 h以后进行体外受精，并收集IVM液，测定超氧化物歧化酶（superoxide dismutase，SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）的活性及脂质过氧化产物丙二醛（monochrome display adapter，MDA）的含量；用酶联免疫法测定雌二醇（estradiol，E₂）和孕酮（progesterone，P₄）的浓度。研究结果表明，与对照组相比，在IVM液中添加0.5 μmol/L RES显著提高卵裂率（P<0.05），但对受精率和囊胚率没有显著影响（P>0.05）；5.0 μmol/L RES显著降低受精率、卵裂率和囊胚率（P<0.05），对胚胎发育有抑制作用；在IVM和体外培养（in vitro culture，IVC）液中分别添加0.5 μmol/L RES均显著提高受精率、卵裂率和囊胚率（P<0.05）。与对照组相比，在IVM液中添加RES对卵丘细胞分泌E₂有一定的抑制作用，5.0 μmol/L RES显著降低E₂浓度（P<0.05）；0.5 μmol/L RES显著提高卵丘细胞P₄分泌量（P<0.05）。0.5和2.0 μmol/L RES增加SOD和GSH-Px等酶的活性，但与对照组相比无显著差异（P>0.05），却显著降低MDA含量（P<0.05）；而5.0 μmol/L RES显著降低抗氧化酶活性并增加MDA含量（P<0.05）。综上所述，在IVM和IVC液中同时添加0.5 μmol/L RES，通过增强卵母细胞抗氧化能力和P₄的浓度，并降低MDA含量，从而提高胚胎卵裂率和囊胚率。

Effects of Resveratrol on in vitro Fertilization of Sheep Oocytes

The purpose of this study was to investigate the effects of resveratrol (RES) at different concentrations (0,0.5,2.0,5.0 μmol/L) on in vitro fertilization (IVF) and antioxidant capacity of ovine oocytes and the secretion of steroid hormones by cumulus cells.Sheep oocytes were fertilized in vitro after maturated in different concentrations of RES for 24 h,and the in vitro maturation (IVM) medium was collected for detecting the enzyme activity of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and the content of monochrome display adapter (MDA).The ELISA method was used to detect the concentration of estradiol (E₂) and progesterone (P₄).The results showed that when compared to the control group,adding 0.5 μmol/L RES to the IVM medium significantly increased the cleavage rate (P<0.05),but had no significant effect on the fertilization rate and blastocyst rate (P>0.05);5.0 μmol/L RES significantly reduced the fertilization rate,cleavage rate and blastocyst rate (P<0.05),which had an inhibitory effect on embryonic development.Adding 0.5 μmol/L RES to IVM and IVC (in vitro culture,IVC) medium significantly increased the fertilization rate,cleavage rate and blastocyst rate (P<0.05).Compared to the control group,the addition of RES to IVM solution had a certain inhibitory effect on the secretion of E₂ by cumulus cells,5.0 μmol/L RES significantly reduced E₂ concentration (P<0.05);0.5 μmol/L RES significantly increased the P₄ secretion of cumulus cells (P<0.05).0.5 and 2.0 μmol/L RES increased the activity of SOD,GSH-Px and other enzymes,but there was no significant difference when compared with the control group (P>0.05),but significantly reduced MDA content (P<0.05),while 5.0 μmol/L RES significantly reduced the activity of antioxidant enzymes and increased the content of MDA (P>0.05).In conclusion,0.5 μmol/L RES simultaneously added to IVM and IVC medium could enhance the antioxidant capacity of oocytes and concentration of P₄,and reduced the content of MDA,thus improved the cleavage rate and blastocyst rate.