| 福州番茄黄化曲叶病毒的全基因组结构特征 |
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| 引用本文： | 张洁,林文武,宛柏杰,余小芳,吴祖建.福州番茄黄化曲叶病毒的全基因组结构特征[J].福建农林大学学报(自然科学版),2016(5):501-504. |
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| 作者姓名： | 张洁 林文武 宛柏杰 余小芳 吴祖建 |
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| 作者单位： | 1. 福建农林大学植物病毒研究所/福建省植物病毒学重点实验室,福建 福州,350002;2. 拜耳作物科学(中国)有限公司,浙江 杭州,310018 |
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| 基金项目： | 国家自然科学基金青年项目(31301640、31301642)，福建省教育厅科技项目(JA13103)，福建农林大学博士后项目(132300117) |
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| 摘 要： | 为明确引起福建省福州市长乐地区番茄上曲叶症状的病原,提取样品总DNA,利用简并引物PA/PB进行PCR扩增,从10个样品中均可扩增到500 bp左右的特异条带.NCBI BLAST序列比对表明,该序列与番茄黄化曲叶病毒(TYLCV)核苷酸序列相似性最高,达99.0%.再根据已知500 bp序列设计1对反向引物,扩增TYLCV分离物Fz01全基因组近全长,克隆并测序.经序列拼接发现,TYLCV分离物Fz01基因组全长2781 nts(KP685598),与已报道的TYLCV其他各分离物相似性均在89.3%以上,而与来自中国不同地区的TYLCV分离物的相似性均在98.7%以上.因此,Fz01属于TYLCV的一个分离物.
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| 关 键 词： | 福州 番茄黄化曲叶病毒 全基因组 序列分析 |
Molecular characterization of Tomato yellow leaf curl virus in Fuzhou |
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| Abstract: | To identify pathogens that caused tomato leaf curl symptoms in Changle area of Fuzhou, Fujian Province, total DNA was extracted from 10 infected samples. By using degenerate primers PA/PB, 500 bp fragments were obtained from all samples. Se-quence analysis showed nucleotide sequences of all fragments showed the highest identity (99.0%) with that of Tomato yellow leaf curl virus ( TYLCV) . To get the complete nucleotide sequence of virus isolate Fz01, a pair of reverse primers was designed according to known genome sequence. Results showed that isolate Fz01 comprised of 2781 nucleotides, reaching 89.3% identity with other iso-lates of TYLCV from GenBank, and more than 98.7% identity with other isolates of TYLCV from China. Therefore, the virus that in-fected tomato in Fuzhou was an isolate of TYLCV. |
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| Keywords: | Fuzhou Tomato yellow leaf curl virus complete genome sequence analysis |
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