金针菇JA/SA信号通路基因鉴定及其对外源JA/SA应答

本研究鉴定了金针菇茉莉酸(JA)信号通路的4个关键基因:COI1、PDF1.2、MYC2-1、MYC2-2与水杨酸(SA)信号通路的4个关键基因:PR1-1、PR1-2、NPR1-1、NPR1-2。NBT染色法和荧光定量结果表明,金针菇JA/SA信号通路可应答外源JA/SA,50μmol·L~(-1)外源JA和500μmol·L~(-1)外源SA处理金针菇菌丝12h可显著提高JA/SA信号通路基因的转录水平,基因PDF1.2响应JA诱导最明显,可作为JA信号通路标记基因;JA/SA信号转导途径间存在协同或拮抗作用:SA信号转导通路中NPR1蛋白对JA信号转导通路中PDF1.2基因表达有抑制作用,外源JA/SA的相对浓度决定其作用的强弱。

Identification of Genes in JA/SA Signaling Pathways in Flammulina velutipes and Their Response to Exogenous JA/SA

In this study,we identified 4 key genes of JA signaling pathway (COI 1、PDF 1.2、MYC2-1、MYC2-2) and SA signaling pathway (PR1-1、PR1-2、NPR1-1、NPR1-2 )in Flammulina velutipes ,respectively.The results of NBT staining and RT-qPCR demonstrated that JA/SA signaling pathways of F .velutipes responsed to exogenous JA/SA.In addition,the expression levels of these genes of JA/SA signaling pathway significantly increased under the treatment of exogenous 50 μmol · L-1 JA and 500 μmol · L-1 SA for 12 h,respectively.It was worth noting that PDF 1.2 could be considered as the marker gene of JA signaling pathways for its response to exogenous JA obviously. The antagonistic effect between JA and SA signaling pathways may also exist in F.velutipes and the protein NPR1 of SA signaling pathways had inhibitory effect on the gene PDF 1.2 expression level of JA signaling pathways which could be affected by the relative concentration of exogenous JA/SA.