龙眼TFL1基因的克隆与表达

以‘红核子’龙眼叶芽为材料,克隆了龙眼TFL1-1和TFL1-2基因的c DNA序列和基因组DNA序列,进行序列分析,并对这两个基因在花芽分化过程中的表达进行了研究.结果显示,龙眼TFL1-1基因c DNA开放阅读框共519 bp,编码173个氨基酸,TFL1-2基因c DNA开放阅读框共525 bp,编码175个氨基酸;两个基因DNA序列均含有4个外显子和3个内含子;序列分析和系统进化树分析表明,龙眼TFL1-1和TFL1-2都是TFL1同源基因,龙眼TFL1-1基因与柑橘、梨的TFL1基因亲缘关系较近;基因表达结果表明,龙眼TFL1-1和TFL1-2基因的功能都与抑制花芽分化有关.

Cloning and expression analysis of longan TFL1 genes

To investigate genes that regulate flower bud differentiation, cDNA and genomic DNA sequences of longan terminal flower 1-1 ( TFL1-1) and terminal flower 1-2 ( TFL1-2) genes were cloned from longan cultivar ‘Honghezi’ , and followed by sequence and expression analysis via PCR. Results showed that TFL1-1 gene had an open reading frame ( ORFs) of 519 bp in length, which encoded 173 amino acid residues, and TFL1-2 gene had an ORF of 818 bp in length, which encoded 175 amino acid residues. And both sequences consisted of 4 exons and 3 introns. Sequence analysis and cluster analysis showed that two longanTFL1 proteins be-longed to TFL1 homologs, and TFL1-1 had closer genetic relationship with citrus TFL1 and pear TFL1. To summarize, both TFL1-1 and TFL1-2 inhibited flower bud differentiation.