H_2S处理对小麦形态及生理生化指标的影响

采用硫氢化钠作为硫化氢的外源性供体,研究不同浓度硫氢化钠(0、0.05、0.10、0.20、0.40、0.80、1.60、3.20mmol·L~(-1))对小麦幼苗形态、生理生化的影响,以探寻H_2S对小麦幼苗生长发育的机制。结果表明,低浓度硫氢化钠处理促进了小麦幼苗的生长,表现为株高、根长、鲜重和干重的增加;而高浓度的硫氢化钠处理则明显抑制了小麦幼苗的生长,甚至伤害,表现为株高、根长、鲜重和干重的减少。同样,不同浓度的硫氢化钠处理也显著引起了植株的含水量、叶片的叶绿素含量的变化。运用主成分法分析发现,小麦幼苗生物量、生理指标等数据之间存在着显著相关性。

Effect of H2S on Morphology and Physiological/Biochemical Properties of Wheat

NaHS was used as an exogenous H2 S for this study to induce changes on the protein expression and morphological/physiological properties of wheat seedlings.Varied concentrations of NaHS (i.e.,0,0.05,0.10, 0.20,0.40,0.80,1.60,and 3.20 mmol·L-1 )were incorporated in the medium solution for the experimentation. According to the effect on the seedling growth and based on their concentrations,3 groups of the solutions were classified as (a)LC at 0.05 and 0.10 mmol·L-1 that promoted the growth,(b)IC at 0.20,1.60,and 0.40 mmol ·L-1 that inhibited the growth,and (c)HC at 0.80 and 3.20 mmol·L-1 that harmed the growth.When LC was applied,the height,root length,and fresh or dry weight of the plants increased significantly,while the application of HC produced the opposite effects.Furthermore,moisture and leaf chlorophyll contents of the plants were significantly affected by the NaHS concentration.There were also significant correlations observed between the biomass and physiological characteristics,according to a principal component analysis.